RT Journal Article
T1 RGS4 RNA Secondary Structure Mediates Staufen2 RNP Assembly in Neurons
A1 Fernández-Moya, Sandra M.
A1 Ehses, Janina
A1 Bauer, Karl E.
A1 Schieweck, Rico
A1 Chakrabarti, Anob M.
A1 Lee, Flora C. Y.
A1 Illig, Christin
A1 Luscombre, Nicholas M.
A1 Harner, Max
A1 Ule, Jernej
A1 Kiebler, Michael A.
K1 Proteínas
AB NA-binding proteins (RBPs) act as posttranscriptional regulators controlling the fate of target mRNAs. Unraveling how RNAs are recognized by RBPs and in turn are assembled into neuronal RNA granules is therefore key to understanding the underlying mechanism. While RNA sequence elements have been extensively characterized, the functional impact of RNA secondary structures is only recently being explored. Here, we show that Staufen2 binds complex, long-ranged RNA hairpins in the 3′-untranslated region (UTR) of its targets. These structures are involved in the assembly of Staufen2 into RNA granules. Furthermore, we provide direct evidence that a defined Rgs4 RNA duplex regulates Staufen2-dependent RNA localization to distal dendrites. Importantly, disrupting the RNA hairpin impairs the observed effects. Finally, we show that these secondary structures differently affect protein expression in neurons. In conclusion, our data reveal the importance of RNA secondary structure in regulating RNA granule assembly, localization and eventually translation. It is therefore tempting to speculate that secondary structures represent an important code for cells to control the intracellular fate of their mRNAs.
PB MDPI
YR 2021
FD 2021-12-01
LK https://hdl.handle.net/10630/45510
UL https://hdl.handle.net/10630/45510
LA eng
NO Fernández-Moya, S.M.; Ehses, J.; Bauer, K.E.; Schieweck, R.; Chakrabarti, A.M.; Lee, F.C.Y.; Illig, C.; Luscombe, N.M.; Harner, M.; Ule, J.; et al. RGS4 RNA Secondary Structure Mediates Staufen2 RNP Assembly in Neurons. Int. J. Mol. Sci. 2021, 22, 13021. https://doi.org/10.3390/ijms222313021
NO Deutsche Forschungsgemeinschaft
NO Friedrich Baur Stiftung
NO The Wellcome Trust
NO Cancer Research UK
NO UK Medical Research Council
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 4 mar 2026