RT Journal Article
T1 Ethylene is involved in strawberry fruit ripening in an organ-specific manner
A1 Merchante-Berg, Catharina
A1 Vallarino Castro, José G.
A1 Osorio-Algar, Sonia
A1 Aragüez, Irene
A1 Villarreal, Natalia
A1 Ariza, María T.
A1 Martínez, Gustavo A.
A1 Medina-Escobar, Nieves
A1 Civello, Marcos P.
A1 Fernie, Alisdair R
A1 Botella, Miguel A.
A1 Valpuesta-Fernández, Victoriano
K1 Fresas -  Efectos del etileno
AB The fruit of the strawberry F ananassa has traditionally been classified as non-climacteric because its ripening process is not governed by ethylene. However, previous studies have reported the timely endogenous production of minor amounts of ethylene by the fruit as well as the differential expression of genes of the ethylene synthesis, reception, and signalling pathways during fruit development. Mining of the F vesca genome allowed for the identification of the two main ethylene biosynthetic genes, ACC synthase and ACC oxidase. Their expression pattern during fruit ripening was found to be stage and organ (achene or receptacle) specific. Strawberry plants with altered sensitivity to ethylene could be employed to unravel the role of ethylene in the ripening process of the strawberry fruit. To this end, independent lines of transgenic strawberry plants were generated that overexpress the Arabidopsis etr1-1 mutant ethylene receptor, which is a dominant negative allele, causing diminished sensitivity to ethylene. Genes involved in ethylene perception as well as in its related downstream processes, such as flavonoid biosynthesis, pectin metabolism, and volatile biosynthesis, were differently expressed in two transgenic tissues, the achene and the receptacle. The different transcriptional responsiveness of the achene and the receptacle to ethylene was also revealed by the metabolic profiling of the primary metabolites in these two organs. The free amino acid content was higher in the transgenic lines compared with the control in the mature achene, while glucose and fructose, and citric and malic acids were at lower levels. In the receptacle, the most conspicuous change in the transgenic lines was the depletion of the tricarboxylic acid cycle intermediates at the white stage of development, most probably as a consequence of diminished respiration. The results are discussed in the context of the importance of ethylene during strawberry fruit ripening.
PB Oxford University Press [University Publisher], Society for Experimental Biology [Society Publisher]
YR 2013
FD 2013-11
LK https://hdl.handle.net/10630/29551
UL https://hdl.handle.net/10630/29551
LA spa
NO Merchante C, Vallarino JG, Osorio S, Aragüez I, Villarreal N, Ariza MT, Martínez GA, Medina-Escobar N, Civello MP, Fernie AR, Botella MA, Valpuesta V. Ethylene is involved in strawberry fruit ripening in an organ-specific manner. J Exp Bot. 2013 Nov;64(14):4421-39. doi: 10.1093/jxb/ert257. Epub 2013 Oct 5. PMID: 24098047; PMCID: PMC3808323.
NO This work was supported by the Ministerio de Ciencia e Innovación (MICINN, Spain) [grant nos BIO2010-15630 and AR2009-0004]. CM was supported by a FPI fellowship from Junta de Andalucía (Spain), and IA by a FPU fellowship (MICINN, Spain). SO and NME acknowledge the support by Ministerio de Ciencia e Innovación, Spain (Ramón and Cajal contract).
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 21 ene 2026