RT Journal Article
T1 An ex vivo Approach in European Seabass Leucocytes Supports the in vitro Regulation by Postbiotics of Aip56 Gene Expression of Photobacterium damselae subsp. piscicida
A1 Domínguez-Maqueda, Marta
A1 Espinosa-Ruiz, Cristóbal
A1 Esteban, María Ángeles
A1 Alarcón, Francisco Javier
A1 Tapia-Paniagua, Silvana Teresa
A1 Balebona-Accino, María del Carmen
A1 Moriñigo-Gutiérrez, Miguel Ángel
K1 Apoptosis
K1 Acuicultura - Agentes patógenos
K1 Peces - Agentes patógenos
K1 Agentes antiinfecciosos
AB Shewanella putrefaciens Pdp11 (SpPdp11) is a probiotic strain assayed in aquaculture; however, its postbiotic potential is unknown. Postbiotics are bacterial metabolites, including extracellular products (ECPs) that improve host physiology and immunity. Their production and composition can be affected by different factors such as the growing conditions of the probiotics. Photobacterium damselae subsp. piscicida strain Lg 41/01 (Phdp) is one of the most important pathogens in marine aquaculture. The major virulent factor of this bacterium is the exotoxin aip56, responsible for inducing apoptosis of fish leucocytes. Viable SpPdp11 cells have been reported to increase resistance to challenges with Phdp. This work aimed to evaluate the effect of two ECPs, T2348-ECP and FM1548-ECP, obtained from SpPdp11 grown under different culture conditions that previously demonstrated to exert different degradative and non-cytotoxic activities, as well as the effect on pathogens biofilm formation. These SpPdp11-ECPs were then analyzed by their effect on the viability, phagocytosis, respiratory burst and apoptogenic activity against European sea bass leucocytes infected or not with Phdp supernatant. Both ECPs, T2348-ECP and FM1548-ECP, were not cytotoxic against leucocytes and significantly reduced their apoptosis. Phagocytosis and respiratory burst of leucocytes were significantly reduced by incubation with Phdp supernatant, and not influenced by incubation with T2348-ECP or FM1548-ECP. However, both activities were significantly increased after leucocyte incubation with combined T2348-ECP and FM1548-ECP with Phdp supernatant, compared to those incubated only with Phdp supernatant. Finally, both T2348-ECP and FM1548-ECP significantly reduced the relative in vitro expression of the Phdp aip56 encoding gene.
PB Springer
YR 2024
FD 2024-04-23
LK https://hdl.handle.net/10630/31166
UL https://hdl.handle.net/10630/31166
LA eng
NO Domínguez-Maqueda, M., Espinosa-Ruíz, C., Esteban, M.Á. et al. An ex vivo Approach in European Seabass Leucocytes Supports the in vitro Regulation by Postbiotics of Aip56 Gene Expression of Photobacterium damselae subsp. piscicida. Probiotics & Antimicro. Prot. (2024). https://doi.org/10.1007/s12602-024-10255-x
NO Funding for open access publishing: Universidad Málaga/CBUA. This work was funded by the proyectos de investigación PID2020-113637RB-C21 and PID2020-113637RB-C22 funded by MCIN/AEI/10.13039/ 501100011033 and is part of the ThinkInAzul programme supported by MCIN with funding from European Union Next Generation EU (PRTR-C17. I1) and by the Comunidad Autónoma de la Región de Murcia-Fundación Séneca (Spain).
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 26 ene 2026