RT Conference Proceedings
T1 Search of candidate genes involved in branched-chain amino acid metabolism in strawberry: functional validation
A1 Romero, Helena
A1 Pott, Delphine
A1 Segado, Enrique
A1 Vallarino Castro, José G.
A1 Osorio-Algar, Sonia
K1 Fresas
K1 Aminoácidos
K1 Transporte
AB Cultivated strawberry (Fragaria × ananassa) is a berry crop widely appreciated worldwide due to itsnutritional and organoleptic properties. However, the main breeding targets in these crops have beenthe control of flowering (for fruit yield), runnering (for vegetative propagation) and the trade-offbetween the two. For this reason, nowadays, breeders are mainly focused on recovering fruit qualityattributes in order to gain consumers acceptance. Those characteristics are determined by themetabolic composition of the fruit, thus the modulation of its metabolism can help to reach this aim.Regarding aroma, esters are the main responsible of this trait in strawberry fruit. Some of them arederived from the catabolism of branched-chain amino acids (valine, leucine and isoleucine). Althoughthe synthesis of these amino acids has been well stablished in strawberry fruit, its degradation remainsunclear. Using a F1 F. × ananassa mapping population we identified a stable QTL (Quantitative TraitLoci) for these amino acids. Based on its differential gene expression in contrasting lines for thesemetabolites and its annotation, we identified an amino acid transporter as a candidate gene for theregulation of branched-chain amino acid content in strawberry. To elucidate the involvement of thisgene in branched-chain amino acid metabolism, we have done functional analysis based on transientoverexpression lines on Nicotiana benthamiana showing changes in amino acids content. Currently,we are performing further experiments in order to elucidate the role of this gene on strawberry volatilemetabolism.
YR 2022
FD 2022
LK https://hdl.handle.net/10630/25319
UL https://hdl.handle.net/10630/25319
LA eng
NO Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech.
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 20 ene 2026