RT Journal Article
T1 Efficient method for rapid multiplication of clean and healthy willow clones via in vitro propagation with broad genotype applicability
A1 Palomo-Ríos, Elena
A1 Macalpine, William
A1 Shield, Ian
A1 Amey, Joanna
A1 Karaoglu, Cuma
A1 West, Jevon
A1 Hanley, Steven
A1 Krygier, Richard
A1 Karp, Angela
A1 Jones, Huw
K1 Microorganismos - Propagación
AB Willow is a versatile crop with considerable potential as a source of renewable biomass for bioenergy. Although breeding new varieties takes less time compared with some other tree species, producing new willow varieties is still a slow, labour-intensive process, partly because clonally propagating the results of each cross is a bottleneck early in the breeding scheme. In this paper, we describe a facile, rapid method for the in vitro culture of a wide range of willow genotypes. We have developed a combination of media and methods for efficient tissue-culture propagation to rapidly multiply individual plants and simultaneously produce clean, stock germplasm applicable to a wide range of willow genotypes that can be phytosanitary tested to demonstrate their disease-free status. The micropropagation method described could generate in the order of 5000 viable, transplantable clones from a single plant in just 24 weeks and was used to produce phytosanitary tested breeding material for export to overcome restriction on the international transport of woody cuttings. This method could represent a valuable biotechnology adjunct to willow breeding programmes and could accommodate early selection via molecular or biochemical markers.
PB Canadian Science Publishing
YR 2015
FD 2015-09-04
LK https://hdl.handle.net/10630/33486
UL https://hdl.handle.net/10630/33486
LA eng
NO Elena Palomo-Ríos, William Macalpine, Ian Shield, Joanna Amey, Cuma Karaoğlu, Jevon West, Steven Hanley, Richard Krygier, Angela Karp, and Huw D. Jones. 2015. Efficient method for rapid multiplication of clean and healthy willow clones via in vitro propagation with broad genotype applicability. Canadian Journal of Forest Research. 45(11): 1662-1667. https://doi.org/10.1139/cjfr-2015-0055
NO Financiado por la BBSRC
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 20 ene 2026