RT Journal Article
T1 Characterizing the involvement of FaMADS9 in the regulation of strawberry fruit receptacle development
A1 Vallarino Castro, José G.
A1 Merchante-Berg, Catharina
A1 Sánchez-Sevilla, José F
A1 De Luis Balaguer, María Angels
A1 Pott, Delphine
A1 Ariza Fernández, María Teresa
A1 Casañal, Ana
A1 Posé-Padilla, David
A1 Vioque, Amalia
A1 Amaya Saavedra, Iraida
A1 Willmitzer, Lothar
A1 Solano, Roberto
A1 Sozzani, Rosangela
A1 Fernie, Alisdair R
A1 Botella-Mesa, Miguel Ángel
A1 Giovannoni, James J.
A1 Valpuesta-Fernández, Victoriano
A1 Osorio-Algar, Sonia
K1 Fresas
AB FaMADS9 is the strawberry (Fragaria x ananassa) gene that exhibits the highest homology to the tomato (Solanum lycopersicum) RIN gene. Transgenic lines were obtained in which FaMADS9 was silenced. The fruits of these lines did not show differences in basic parameters, such as fruit firmness or colour, but exhibited lower Brix values in three of the four independent lines. Thegene ontology MapMan category that was most enriched among the differentially expressed genes in the receptacles at the white stage corresponded to the regulation of transcription, including a high percentage of transcription factors and regulatory proteins associated with auxin action. In contrast, the most enriched categories at the red stage were transport, lipid metabolism and cell wall. Metabolomic analysis of the receptacles of the transformed fruits identified significant changes in the content of maltose, galactonic acid-1,4-lactone, proanthocyanidins and flavonols at the green/white stage, while isomaltose, anthocyanins and  cuticular wax metabolism were the most affected at the red stage. Among the regulatory genes that were differentially expressed in the transgenic receptacles were several genes previously linked to flavonoid metabolism, such as MYB10, DIV, ZFN1, ZFN2, GT2, and GT5, or associated with the action of hormones, such as abscisic acid, SHP, ASR, GTE7 and SnRK2.7. The inference of a generegulatory network, based on a dynamic Bayesian approach, among the genes differentially expressed in the transgenic receptacles at the white and red stages, identified the genes KAN1, DIV, ZFN2 and GTE7 as putative targets of FaMADS9. A MADS9-specific CArG box was identified in the promoters of these genes.
PB Wiley Online Library
YR 2019
FD 2019-09-18
LK https://hdl.handle.net/10630/23600
UL https://hdl.handle.net/10630/23600
LA eng
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 20 ene 2026