RT Journal Article
T1 Influence of pore size in protein G'-grafted mesoporous silica nanoparticles as a serum pretreatment system for in vitro allergy diagnosis
A1 Paris, Juan Luis
A1 Monío, Cristina
A1 Pérez-Moreno, Ana M.
A1 Jurado-Escobar, Raquel
A1 Bogas Herrera, Gádor
A1 Fernández-Duarte, Tahía Diana
A1 Montañez-Vega, María Isabel
A1 Mayorga Mayorga, Cristobalina
A1 Torres-Jaén, María Josefa
K1 Alergia-Diagnóstico
AB Particles with the capacity to bind to immunoglobulin G (IgG) can be used for the purification of IgG or to process clinical samples for diagnostic purposes. For in vitro allergy diagnosis, the high IgG levels in serum can interfere with the detection of allergen-specific IgE, the main diagnostic biomarker. Although commercially available, current materials present a low IgG capture capacity at large IgG concentrations or require complex protocols, preventing their use in the clinic. In this work, mesoporous silica nanoparticles are prepared with different pore sizes, to which IgG-binding protein G’ is grafted. It is found that for one particular optimal pore size, the IgG capture capacity of the material is greatly enhanced. The capacity of this material to efficiently capture human IgG in a selective way (compared to IgE) is demonstrated in both solutions of known IgG concentrations as well as in complex samples, like serum, from healthy controls and allergic patients using a simple and fast incubation protocol. Interestingly, IgG removal using the best-performing material enhances in vitro IgE detection in sera from patients allergic to amoxicillin. These results highlight the great translation potential of this strategy to the clinic in the context of in vitro allergy diagnosis.
PB Wiley
YR 2023
FD 2023
LK https://hdl.handle.net/10630/26282
UL https://hdl.handle.net/10630/26282
LA eng
NO Paris JL, Monío C, Pérez-Moreno AM, Jurado-Escobar R, Bogas G, Fernández TD, Montañez MI, Mayorga C, Torres MJ. Influence of Pore Size in Protein G'-Grafted Mesoporous Silica Nanoparticles as a Serum Pretreatment System for In Vitro Allergy Diagnosis. Adv Healthc Mater. 2023 Feb 27:e2203321. doi: 10.1002/adhm.202203321. Epub ahead of print. PMID: 36847336.
NO Funding for Open Access charge: Universidad de Málaga/CBUA. TEM experimentswere performed in the ICTS “NANBIOSIS,” more specifically in the U28Unit at IBIMA Plataforma BIONAND.
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 19 ene 2026