RT Conference Proceedings
T1 Laryngeal effects of stimulation of the cuneiform nucleus (CnF) in spontaneously breathing anaesthetized rats
A1 Carrillo-Franco, Laura
A1 López-González, Manuel Víctor
A1 González-García, Marta
A1 Díaz-Casares, Amelia
A1 Dawid-Milner, Marc Stefan
K1 Neuronas motoras
K1 Vocalización
K1 Laringe
AB The aim of this study was to characterise the relationship between mesencephalic-pontine neuronal circuits to understand their role in laryngeal control and its effect on vocalization. Methods: Experimental studies were carried out with non-inbred male rats (n=7), SPF, Sprague-Dawley (300-350 g) housed under standard conditions. Animals were anesthetized with sodium pentobarbitone (60 mg/kg i.p., initial dose, supplemented 2 mg/ kg, i.v., as necessary). A double tracheal cannulation (upwards in direction of the glottis for the “glottis isolated in situ” technique, and downwards in the direction of the carina) was done. Subglottic pressure was recorded with a precision differential pressure transducer (ADInstrument model FE141, ± 0,03 psi) by passing a stream of humidified medical air upwards through the larynx at a constant rate of 30-70 ml/min with a thermal mass digital air flow meter controller (Bronkhorst Hi-Tec F-201CV-AGD-22-V). Electrical stimulation of the CnF using concentric bipolar electrodes (1 ms pulses, 20-40 µA, 100 Hz for 5 s) was performed. Respiratory flow, pleural pressure, blood pressure, heart rate and ECG activity were also recorded. Results: CnF stimulation evoked a decrease of laryngeal resistance (subglottal pressure) (p<0,01) accompanied with an inspiratory facilitatory response consisted of an increase in respiratory rate (p<0,01), together with a pressor (p<0,001) and a tachycardic response (p<0,01).Conclusions: The results of our study contribute with new data on the role of the CnF in the mechanisms controlling subglottic pressure and laryngeal activity.
YR 2021
FD 2021-11
LK https://hdl.handle.net/10630/23206
UL https://hdl.handle.net/10630/23206
LA spa
NO Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech.
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 22 ene 2026