RT Conference Proceedings
T1 Human Mesenchymal Stem Cells and its Exosomes in Posthemorrhagic Hydrocephalus: A Focus on Edema and Ependymal Repair
A1 López-de-San-Sebastián, Javier
A1 Rodríguez-Pérez, Luis Manuel
A1 Anguita-Guardia, Alba
A1 Jiménez-Lara, Antonio Jesús
A1 Páez-González, Patricia
K1 Hidrocefalia
AB Background Germinal matrix hemorrhages and intraventricular hemorrhages (GMH/IVH) often lead to posthemorrhagic hydrocephalus (PHH), a severe cause of morbidity and mortality in premature neonates. GMH/IVH is known to disrupt the ependyma, which forms a functional and physical barrier between the cerebrospinal fluid (CSF) and the brain tissue. Consequently, CSF circulation and physiology are severely affected; and thus, ependyma is a chiefly important target when it comes to designing PHH treatments. Bone marrow-derived mesenchymal stem cells (MSCs) are extremely effective anti-inflammatory agents which have already shown positive results regarding PHH treatment. However, there is no therapy to recover the ependyma in humans.  Methods Human MSCs were cultured under inflammatory or non-inflammatory conditions to extract and purify their correspondent exosomal fraction through sequential centrifugation steps. The effect on the ependymal differentiation and ciliogenesis was tested in vivo and in vitro. Also, the effect on the parenchymal edema was assesed in vivo on a PHH surgical model. Conditioned and non-conditioned exosomes were used. Ventricular wall explants were analysed through confocal microscopy. The exosomes were functionally characterised through proteomics. Results Firstly, no rejection of the human MSCs nor of their exosomes is observed. Both MSCs and exosomes have a posititve effect on ciliogenesis and edema proportion and severiy. This also indicates that exosomes are able to partially reproduce the human MSCs therapeutical effect in vitro and in vivo. Functional enrichment of proteomics gives some insight on the mechanism that makes the therapy possible.
PB SRHSB 2024 Annual Conference
YR 2023
FD 2023-09-04
LK https://hdl.handle.net/10630/37497
UL https://hdl.handle.net/10630/37497
LA eng
NO Junta de AndalucíaInstituo de Salud Carlos III
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 20 ene 2026