RT Conference Proceedings
T1 Brain tissue recovery in obstructive congenital hydrocephalus after intraventricular transplantation of mesenchymal stem cells
A1 García-Bonilla, María
A1 Ojeda-Pérez, Betsaida
A1 Shumilov, Kirill
A1 Vitorica Ferrández, Javier
A1 García-Martín, María Luisa
A1 Muñoz, Carmen
A1 Gutiérrez-Pérez, Antonia
A1 Jiménez-Lara, Antonio Jesús
A1 Páez-González, Patricia
K1 Hidrocefalia
AB Introduction: Bone marrow-derived mesenchymal stem cells (BM-MSC) are a potential therapeutic tool due to their ability for migrating and producing neuroprotector factors when transplanted. The aim of this study was to evaluate the short-time effects of a BM-MSC experimental therapy in the hyh mouse model with severe obstructive hydrocephalus. Methods: BM-MSC were characterized in vitro and then injected into the ventricles of hyh mice. Wild-type and saline-injected hyh mice were used as controls. Samples were studied by analyzing and comparing mRNA, protein and metabolites level expression in control and damaged tissue.Results: Undifferentiated BM-MSC were found to: i) spread into the periventricular astrocyte reaction region after four days post-injection, and, ii) be producing neuroprotector factors (GDNF and VEGF). Astrocytes located in periventricular edematous region increased their aquaporin-4 expression, as well as Slit2 expression (neuroprotective and anti-inflammatory molecule). There was also a significant reduction of osmolytes such as taurine and neuroexcytotoxic glutamate. Halved apoptotic cell death was detected in the periventricular walls.Conclusions: BM-MSC lead to recovery of the severe neurodegenerative conditions associated to congenital hydrocephalus mediated by reactive astrocytes.
YR 2018
FD 2018-10-29
LK https://hdl.handle.net/10630/16737
UL https://hdl.handle.net/10630/16737
LA eng
NO Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech.Supported by Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech, and PI15/0619 (ISCIII/FEDER).
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 22 ene 2026