RT Journal Article
T1 Synchrotron radiation FTIR microspectroscopy enables measuring dynamic cell identity patterning during human 3D differentiation.
A1 Dučić, Tanja
A1 Rodríguez-Yáñez, Francisco
A1 González-Muñoz, María Elena
K1 Fourier, Espectroscopía por transformada de
K1 Biomoléculas
K1 Citología
K1 Embriología
K1 Células - Diferenciación
AB Human cell fate specification, particularly in neural development, is difficultto study due to limited access to embryonic tissues and differences fromanimal models. Human induced pluripotent stem cells (hiPSCs) and 3D organoidmodels enable the study of early human neural development, surpassinglimitations of 2D cultures by incorporating crucial cell-cell and cell-matrixinteractions. In this study, we used synchrotron radiation-based Fouriertransform infrared (SR-FTIR) microspectroscopy to examine biomolecularprofiles of 3D-differentiated organoids, specifically embryoid bodies (EBs) andneural spheroids (NS), derived from hiPSCs. SR-FTIR allowed us to analyzethese organoids’ cellular identity at a biomolecular level, offering a holisticview that complements specific cell markers. Our findings reveal distinctbiomolecular identities in 3D organoids, with differences in DNA structure,lipid saturation, phospholipid composition, and protein conformations. Thisapproach highlights that cellular identity is shaped by more than geneexpression alone; it involves unique biomolecular compositions that can bedetected even in complex, multicellular environments. By demonstrating therole of molecular configuration in cell differentiation, our findings suggestthat differentiation processes extend beyond genetics, involving interdependentbiochemical signals. This study demonstrates the unique efficacy SR-FTIR inanalyzing human-specific 3D models for investigating complex multicellulardifferentiation mechanisms, offering new avenues for understanding thebiochemical basis of human development and disease.
PB Frontiers
YR 2025
FD 2025-05-21
LK https://hdl.handle.net/10630/38819
UL https://hdl.handle.net/10630/38819
LA eng
NO Dučić T, Rodriguez-Yañez F and Gonzalez-Muñoz E (2025) Synchrotron radiation FTIR microspectroscopy enables measuring dynamic cell identity patterning during human 3D differentiation. Front. Cell Dev. Biol. 13:1569187. doi: 10.3389/fcell.2025.1569187
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 20 ene 2026