RT Journal Article
T1 Functional and Structural Analysis Reveals Distinct Biological Roles of Plant Synaptotagmins in Response to Environmental Stress.
A1 García-Hernández, Selene
A1 Rubio-Valverde, Lourdes
A1 Rivera Moreno, María
A1 Pérez-Sancho, Jessica
A1 Morello-López, Jorge
A1 Esteban del Valle, Alicia
A1 Benítez-Fuente, Francisco
A1 Beuzón-López, Carmen del Rosario
A1 Macho, Alberto P.
A1 Ruiz-López, Noemí
A1 Albert, Armando
A1 Botella-Mesa, Miguel Ángel
K1 Estrés (Fisiología)
K1 Plantas - Efectos del estrés
K1 Fisiología vegetal
K1 Arabidopsis - Efectos del estrés
AB Endoplasmic reticulum–plasma membrane contact sites (ER–PM CSs) are evolutionarily conserved membrane domains found inall eukaryotes, where the ER closely interfaces with the PM. This short distance is achieved in plants through the action of tetherproteins such as synaptotagmins (SYTs). Arabidopsis comprises five SYT members (SYT1–SYT5), but whether they possessoverlapping or distinct biological functions remains elusive. SYT1, the best‐characterized member, plays an essential role in theresistance to abiotic stress. This study reveals that the functionally redundant SYT1 and SYT3 genes, but not SYT5, are involved insalt and cold stress resistance. We also show that, unlike SYT5, SYT1 and SYT3 are not required for Pseudomonas syringaeresistance. Since SYT1 and SYT5 interact in vivo via their SMP domains, the distinct functions of these proteins cannot be caused bydifferences in their localization. Interestingly, structural phylogenetic analysis indicates that the SYT1 and SYT5 clades emergedearly in the evolution of land plants. We also show that the SYT1 and SYT5 clades exhibit different structural features in their SMPand Ca2+ binding of their C2 domains, rationalizing their distinct biological role.
PB Wiley
YR 2024
FD 2024-09-10
LK https://hdl.handle.net/10630/40124
UL https://hdl.handle.net/10630/40124
LA eng
NO García-Hernández S, Rubio L, Rivera-Moreno M, Pérez-Sancho J, Morello-López J, Esteban Del Valle A, Benítez-Fuente F, Beuzón CR, Macho AP, Ruiz-López N, Albert A, Botella MA. Functional and Structural Analysis Reveals Distinct Biological Roles of Plant Synaptotagmins in Response to Environmental Stress. Plant Cell Environ. 2025 Jan;48(1):260-271. doi: 10.1111/pce.15125.
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 21 ene 2026