RT Journal Article
T1 Fasciola hepatica Induces Eosinophil Apoptosis in the Migratory and Biliary Stages of Infection in Sheep.
A1 Escamilla-Sánchez, Alejandro
A1 Bautista, María José
A1 Zafra, Rafael
A1 Pacheco Luque, Isabel
A1 Ruiz-Campillo, María Teresa
A1 Martínez-Cruz, Setefilla
A1 Méndez, Aniceto
A1 Martinez Moreno, Álvaro
A1 Molina-Hernández, Verónica
A1 Pérez, José
K1 Ganado ovino - Enfermedades
K1 Ovejas - Inmunología
K1 Ovejas - Hígado - Enfermedades
K1 Fasciola hepática
AB The aim of the present work was to evaluate the number of apoptotic eosinophils in the liversof sheep experimentally infected with Fasciola hepatica during the migratory and biliarystages of infection. Four groups (n = 5) of sheep were used; groups 1-3 were orally infectedwith 200 metacercariae (mc) and sacrificed at 8 and 28 days post-infection (dpi), and 17weeks post-infection (wpi), respectively. Group 4 was used as an uninfected control.Apoptosis was detected using immunohistochemistry with a polyclonal antibody against antiactivecaspase-3, and transmission electron microscopy (TEM). Eosinophils were identifiedusing the Hansel stain in serial sections for caspase-3, and by ultrastructural features usingTEM. At 8 and 28 dpi, numerous caspase-3+ eosinophils were mainly found at the peripheryof acute hepatic necrotic foci. The percentage of caspase -3+ apoptotic eosinophils in theperiphery of necrotic foci was high (46.1 to 53.9) at 8 and 28 dpi, respectively, and decreasedin granulomas found at 28 dpi (6%). Transmission electron microscopy confirmed thepresence of apoptotic eosinophils in hepatic lesions at 8 and 28 dpi. At 17 wpi, apoptoticeosinophils were detected in the infiltrate surrounding some enlarged bile ducts containingadult flukes. This is the first report of apoptosis induced by F. hepatica in sheep and the firststudy reporting apoptosis in eosinophils in hepatic inflammatory infiltrates in vivo. The highnumber of apoptotic eosinophils in acute necrotic tracts during the migratory and biliarystages of infection suggests that eosinophil apoptosis may play a role in F. hepatica survivalduring different stages of infection.
PB Elsevier
YR 2016
FD 2016-01-30
LK https://hdl.handle.net/10630/29132
UL https://hdl.handle.net/10630/29132
LA eng
NO Escamilla, A., Bautista, M. J., Zafra, R., Pacheco, I. L., Ruiz, M. T., Martínez-Cruz, S., Méndez, A., Martínez-Moreno, A., Molina-Hernández, V., & Pérez, J. (2016). Fasciola hepatica induces eosinophil apoptosis in the migratory and biliary stages of infection in sheep. Veterinary parasitology, 216, 84–88.
NO Política de acceso abierto tomada de: https://v2.sherpa.ac.uk/id/publication/12975
NO EU grants (FPVII-265862-PARAVAC, H2020-635408-PARAGONE)Spanish Ministry of Science grant AGL2009-08726
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 19 ene 2026