RT Conference Proceedings
T1 Generation and characterization of human ipscderived neural precursors and astrocytes to model Alzeimer’s disease
A1 Cáceres Palomo, Laura
K1 Alzheimer, Enfermedad de
K1 Sistema nervioso - Degeneración
AB Background: Alzheimer's disease (AD) is characterized by presenting acomplex pathology, not fully resolved yet. This fact, together with the lackof reliable models, has impeded the development of effective therapies.Recently, several studies have shown that functional glial cell defects havea key role in the pathology of AD. However, this glial dysfunction,currently, cannot be correctly modeled using the available animal models,so we hypothesized that cells derived from Alzheimer's patients can serve asa better platform for studying the disease. In this sense, human pluripotentstem cells (hPSC) allow the generation of different types of neural cells,which can be used for disease modeling, identification of new targets anddrugs development.Methods: We have a collection of hiPSCs derived from patients withsporadic forms of AD stratified based on APOE genotype. We havedifferentiated these cells towards neural cells and mature them to neurons orastrocytes using a serum-free approach, to assess intrinsic differencesbetween those derived from AD patients or healthy controls.Results: We have implemented a serum-free approach and generatedneural precursors and astrocytes from all the lines tested. We observedifferences at the phenotypic level and a reduced capacity to differentiatetowards neural lineage in those lines derived from APOE4 carriers.Conclusions: Our preliminary data suggest intrinsic differences in theneural differentiation capacity between cell lines derived from APOE4 orAPOE3 carrier subjects. Further experiments would contribute to elucidatenovel pathogenic pathways associated with neurodegeneration andsusceptible of therapeutic modulation, likely contributing to thedevelopment of new effective drugs against AD.
YR 2022
FD 2022
LK https://hdl.handle.net/10630/25599
UL https://hdl.handle.net/10630/25599
LA eng
NO Acknowledgments:This study was supported by Instituto de Salud Carlos III (ISCiii) of Spain, cofinancedby FEDER funds from European Union, through grants PI21/00915 (toAG) and PI21/00914 (to JV); by Junta de Andalucia through Consejería deEconomía y Conocimiento grants PY18-RT-2233 (to AG) and US-1262734 (to JV)co-financed by Programa Operativo FEDER 2014-2020, Consejeria de Salud grantPI-0276-2018 (to JAGL) and Programa Operativo de Empleo Juvenil SNGJ4-11 toLCP.Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech.
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 23 ene 2026