RT Journal Article
T1 Interaction of surfactant and protein at the O/W interface and its effect on colloidal and biological properties of polymeric nanocarriers.
A1 del Castillo-Santaella, Teresa
A1 Peula-García, José Manuel
A1 Maldonado-Valderrama, Julia
A1 Jódar-Reyes, Ana Belén
K1 Tensión superficial
K1 Polímeros
K1 Nanopartículas
K1 Proteínas
AB Hypothesis: The use of polymer-based surfactants in the double-emulsion (water/oil/water, W/O/W) solventevaporationtechnique is becoming a widespread strategy for preparing biocompatible and biodegradablepolymeric nanoparticles (NPs) loaded with biomolecules of interest in biomedicine, or biotechnology. Thisapproach enhances the stability of the NPs, reduces their size and recognition by the mononuclear phagocyticsystem, and protects the encapsulated biomolecule against losing biological activity. Different protocols to addthe surfactant during the synthesis lead to different NP colloidal properties and biological activity.Experiments: We develop an in vitro model to mimic the first step of the W/O/W NP synthesis method, whichenables us to analyze the surfactant-biomolecule interaction at the O/W interface. We compare the interfacialproperties when the surfactant is added from the aqueous or the organic phase, and the effect of pH of thebiomolecule solution. We work with a widely used biocompatible surfactant (Pluronic F68), and lysozyme,reported as a protein model.Findings: The surfactant, when added from the water phase, displaces the protein from the interface, henceprotecting the biomolecule. This could explain the improved colloidal stability of NPs, and the higher biologicalactivity of the lysozyme released from nanoparticles found with the counterpart preparation.
PB Elsevier B.V.
YR 2018
FD 2018-09-29
LK https://hdl.handle.net/10630/28911
UL https://hdl.handle.net/10630/28911
LA eng
NO Del Castillo-Santaella T, Peula-García JM, Maldonado-Valderrama J, Jódar-Reyes AB. Interaction of surfactant and protein at the O/W interface and its effect on colloidal and biological properties of polymeric nanocarriers. Colloids Surf B Biointerfaces. 2019 Jan 1;173:295-302. doi: 10.1016/j.colsurfb.2018.09.072
NO Financial support granted by the following research projects: MAT2013-43922-R –European FEDER support included–(MICINN, Spain), RYC-2012-10556, MAT2015-63644-C2-1-R and PI12/2956.
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 20 ene 2026