RT Journal Article
T1 Severe acute respiratory syndrome coronavirus E protein transports calcium ions and activates the NLRP3 inflammasome
A1 Nieto-Torres, Jose Luis
A1 Verdiá-Báguena, Carmina
A1 Jimenez-Guardeño, Jose Manuel
A1 Regla-Nava, Jose Angel
A1 Castaño-Rodriguez, Carlos
A1 Fernandez-Delgado, Raul
A1 Torres, Jaume
A1 Aguilella, Vicente M
A1 Enjuanes, Luis
K1 Virulencia (Microbiología)
AB Severe acute respiratory syndrome coronavirus (SARS-CoV) envelope (E) protein is a viroporin involved in virulence. E protein ion channel (IC) activity is specifically correlated with enhanced pulmonary damage, edema accumulation and death. IL-1β driven proinflammation is associated with those pathological signatures, however its link to IC activity remains unknown. In this report, we demonstrate that SARS-CoV E protein forms protein-lipid channels in ERGIC/Golgi membranes that are permeable to calcium ions, a highly relevant feature never reported before. Calcium ions together with pH modulated E protein pore charge and selectivity. Interestingly, E protein IC activity boosted the activation of the NLRP3 inflammasome, leading to IL-1β overproduction. Calcium transport through the E protein IC was the main trigger of this process. These findings strikingly link SARS-CoV E protein IC induced ionic disturbances at the cell level to immunopathological consequences and disease worsening in the infected organism.
PB Elsevier
YR 2015
FD 2015
LK https://hdl.handle.net/10630/36926
UL https://hdl.handle.net/10630/36926
LA eng
NO Jose L. Nieto-Torres, Carmina Verdiá-Báguena, Jose M. Jimenez-Guardeño, Jose A. Regla-Nava, Carlos Castaño-Rodriguez, Raul Fernandez-Delgado, Jaume Torres, Vicente M. Aguilella, Luis Enjuanes, Severe acute respiratory syndrome coronavirus E protein transports calcium ions and activates the NLRP3 inflammasome, Virology, Volume 485, 2015, Pages 330-339, ISSN 0042-6822, https://doi.org/10.1016/j.virol.2015.08.010.
NO The work done by the authors was supported by Grants from the Government of Spain (BIO2013-42869-R, FIS2013-40473-P), Generalitat Valenciana (Prometeo 2012/069), Fundació Caixa Castelló-Bancaixa (P1-1B2012-03) and a U.S. National Institutes of Health (NIH) project (5P01 AI060699). JLN, JMJ and JAR received contracts from NIH. CCR received a contract from Fundacion La Caixa.
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 4 mar 2026