RT Journal Article
T1 A collagen-targeted biomimetic RGD peptide to promote osteogenesis
A1 Visser, Rick
A1 Arrabal-García, Pilar María
A1 Santos-Ruiz, Leonor
A1 Fernández-Barranco, Raúl
A1 Becerra-Ratia, José
A1 Cifuentes-Rueda, Manuel
K1 Huesos - Crecimiento
K1 Tejidos (Biología) - Diferenciación
AB Osteogenesis is a complex, multifactorial process in which many different signals interact. The bone morphogenetic proteins (BMPs) are the most potent inducers of osteoblastic differentiation, although very high doses of BMPs in combination with collagen type I formulations have to be used for clinical applications. Although integrin-binding arginine-glycine-aspartic acid (RGD) biomimetic peptides have shown some promising abilities to promote the attachment of cells to biomaterials and to direct their differentiation, the linking of these peptides to collagen sponges usually implies chemical manipulation steps. In this study, we describe the design and characterization of a synthetic collagen-targeted RGD biomimetic (CBD-RGD) peptide formed from a collagen-binding domain derived from the von Willebrand factor and the integrin-binding RGD sequence. This peptide was demonstrated to bind to absorbable collagen type I sponges (ACSs) without performing any chemical linking, and to induce the differentiation of MC3T3-E1 mouse preosteoblasts and rat bone marrow-derived mesenchymal stem cells. Furthermore, in vivo experiments showed that ACSs functionalized with CBD-RGD and loaded with a subfunctional dose of BMP-2-formed ectopic bone in rats, while nonfunctionalized sponges loaded with the same amount of BMP-2 did not. These results indicate that the combination of this biomimetic peptide with the currently used collagen+BMP system might be a promising approach to improve osteogenesis and to reduce the doses of BMPs needed in clinical orthopedics.
PB MARY ANN LIEBERT, INC
SN 1937-3341
YR 2014
FD 2014-01
LK https://hdl.handle.net/10630/29701
UL https://hdl.handle.net/10630/29701
LA eng
NO Rick Visser, Pilar M. Arrabal, Leonor Santos-Ruiz, Raul Fernandez-Barranco, Jose Becerra, and Manuel Cifuentes.A Collagen-Targeted Biomimetic RGD Peptide to Promote Osteogenesis.Tissue Engineering Part A.Jan 2014.34-44.http://doi.org/10.1089/ten.tea.2012.0610
NO MICINN (BIO2009-13903-C01-01)MINECO (BIO2012-34960)Junta de Andalucía (P07-CVI-2781)ISCiii, RETICS, Red Española de Terapia Celular (TerCel) (RD12/0019/0032)ISCiii, CIBER-BBN (CB06/01/1015)
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 20 ene 2026