RT Journal Article
T1 Wheat Type One Protein Phosphatase Participates in the Brassinosteroid Control of Root Growth via Activation of BES1.
A1 Bradai, Mariem
A1 Amorim-Silva, Vitor
A1 Belgaroui, Nibras
A1 Esteban del Valle, Alicia
A1 Chabouté, Marie-Edith
A1 Schmit, Anne-Catherine
A1 Lozano-Durán, Rosa
A1 Botella-Mesa, Miguel Ángel
A1 Hanin, Moez
A1 Ebel, Chantal
K1 Plantas - Metabolismo
K1 Raíces (Botánica) - Crecimiento
K1 Hormonas vegetales
K1 Fitorreguladores
AB Brassinosteroids (BRs) play key roles in diverse plant growth processes through a complex signaling pathway. Components orchestrating the BR signaling pathway include receptors such as kinases, transcription factors, protein kinases and phosphatases. The proper functioning of the receptor kinase BRI1 and the transcription factors BES1/BZR1 depends on their dephosphorylation by type 2A protein phosphatases (PP2A). In this work, we report that an additional phosphatase family, type one protein phosphatases (PP1), contributes to the regulation of the BR signaling pathway. Co-immunoprecipitation and BiFC experiments performed in Arabidopsis plants overexpressing durum wheat TdPP1 showed that TdPP1 interacts with dephosphorylated BES1, but not with the BRI1 receptor. Higher levels of dephosphorylated, active BES1 were observed in these transgenic lines upon BR treatment, indicating that TdPP1 modifies the BR signaling pathway by activating BES1. Moreover, ectopic expression of durum wheat TdPP1 lead to an enhanced growth of primary roots in comparison to wild-type plants in presence of BR. This phenotype corroborates with a down-regulation of the BR-regulated genes CPD and DWF4. These data suggest a role of PP1 in fine-tuning BR-driven responses, most likely via the control of the phosphorylation status of BES1.
PB MDPI
YR 2021
FD 2021-09-27
LK https://hdl.handle.net/10630/34675
UL https://hdl.handle.net/10630/34675
LA eng
NO Bradai M, Amorim-Silva V, Belgaroui N, Esteban del Valle A, Chabouté M-E, Schmit A-C, Lozano-Duran R, Botella MA, Hanin M, Ebel C. Wheat Type One Protein Phosphatase Participates in the Brassinosteroid Control of Root Growth via Activation of BES1. International Journal of Molecular Sciences. 2021; 22(19):10424. https://doi.org/10.3390/ijms221910424
NO The authors acknowledge the Tunisian Ministry of Higher Education and Research for funding, and the National Agency for Research Promotion (ANPR) for the financial management of the PRIMA Project GENDIBAR. This work was also supported by the Ministerio de Economía y Competitividad, co-financed by the European Regional Development Fund (grant BIO2017-82609-R to M.A.B and V.A-S). Mariem Bradai was granted by Mujeres por Africa and from COST INDEPTH Action (COST CA16212, 2017).
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 1 mar 2026