RT Journal Article
T1 The influence of rehydration technique on the response of recalcitrant seed embryos to desiccation
T2 La influencia de la técnica de rehidratación en la respuesta a la desecación de los embriones de semillas recalcitrantes
A1 Perán-Quesada, Rosa Amelia
A1 Pammenter, Norman W.
A1 Naicker, Janine
A1 Berjak, Patricia
K1 Semillas - Viabilidad
AB The concept of ‘imbibitional damage’ arose when it was observed that considerable leakage of cell contents could occur when dry seed or pollen tissues are plunged directly into water. It is now common practice to imbibe dehydrated tissue slowly, to permit the re-establishment of functional membranes, prior to placing the tissue into liquid water. However, this argument may not hold if the tissue of interest is inherently desiccation-sensitive. Slow drying of desiccation-sensitive (recalcitrant) seeds orexcised embryonic axes results in damage at high water contents, because it permits time for aqueous-based deleterious processes to occur. The same argument may apply if partially dried material is re-imbibed slowly, as this technique will also expose the tissue to intermediate water contents for protracted periods. This hypothesis was tested using embryos or axes from seeds of three recalcitrant species (Artocarpus heterophyllus, Podocarpus henkelii and Ekebergia capensis). Excisedmaterial was rapidly dried to water contents within the range over which viability is lost during drying, and re-imbibed either rapidly, by plunging directly into water, or slowly, by placing the material on damp filter paper or exposing it to a saturated atmosphere for several hours. Although details of the response differed among species and developmental stage, in all cases direct re-imbibition in water resulted in higher (or similar, but never lower) survival than either of the slow rehydration techniques.
PB Cambridge University Press
YR 2004
FD 2004
LK https://hdl.handle.net/10630/37335
UL https://hdl.handle.net/10630/37335
LA eng
NO Perán R, Pammenter NW, Naicker J, Berjak P. The influence of rehydration technique on the response of recalcitrant seed embryos to desiccation. Seed Science Research. 2004;14(2):179-184. doi:10.1079/SSR2004167
NO Según se especifica en :https://www.cambridge.org/core/services/open-research/preprint-policy: A ‘preprint’ is an early version of a manuscript, created prior to the version submitted for publication. Cambridge's preprint policy permits you to share any pre-submission version of your manuscript anywhere, at any time, under any licence.It is best practice to link preprints to the final published work (the ‘Version of Record’). If you have posted your preprint online, we encourage you to cite your preprint in your submitted manuscript, including details such as a DOI or other persistent identifier. If your manuscript is accepted and published by Cambridge, we also encourage you to update your preprint’s records to link it to your final published Version of Record, using a DOI and URL to link to the version published by Cambridge.(DOI)
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 4 mar 2026