RT Conference Proceedings
T1 Contribution of Xenopus model to a better understanding of cardiac outflow tract
A1 Torres-Prioris, Agustina María
A1 Smith, Stuart J
A1 Mohun, Timothy J.
A1 Fernández, Borja
A1 Durán, Ana C.
K1 Corazón - Enfermedades
K1 Animales de laboratorio
AB Contribution of Xenopus model to a better understanding of cardiac outflow tract.A Torres-Prioris 1, SJ Smith 2, TJ Mohun 2, B Fernández 1, AC Durán 1.1 Department of Animal Biology, Faculty of Science, and Biomedical Research Institute of Málaga (IBIMA), University of Málaga, Spain.2 Developmental Biology Division, The Francis Crick Institute, Mill Hill Laboratory, London, UK.The morphology and morphogenesis of the cardiac outflow tract is a major topic in the study of the vertebrate circulatory system, especially regarding the pathologies affecting this region in humans. Recent studies have demonstrated that, in fish, the cardiac outflow tract consists of a myocardial conus arteriosus and a nonmyocardial bulbus arteriosus. Moreover, the bulbus arteriosus of fish has been considered homologous to the intrapericardial base of the aortic andpulmonary trunks of birds and mammals. Under this perspective, we have conducted a study on the outflow tract of Xenopus laevis, using histological, immunohistochemical and 3D reconstruction techniques. It has been assumedthat the outflow tract of Xenopus, which is intercalated between the ventricle and the great arterial trunks, is of myocardial nature. At its luminal side, it contains two sets of valves between which the so-called spiral valve lies.Our results demonstrate that, together with a proximal myocardial segment, a distal, nonmyocardial, intrapericardial segment is also present in amphibians. We propose that this distal segment, from which the pulmocutaneous andsystemic arteries arise, is homologous to the bulbus arteriosus of fish. Therefore, the bulbus arteriosus is an evolutionarily conserved structure, which has become the aortic and pulmonary roots of birds and mammals. Our findingscontribute to strengthening Xenopus as a good model to better understand the outflow tract morphology and evolution, and as an emerging model for studying human congenital heart diseases.This work was supported by CGL2010-16417, BES-2011-046901, Estancias Breves para FPI (2012, 2013) and FEDER funds.
PB SAGE Publications Press Ldt
YR 2015
FD 2015-11
LK http://hdl.handle.net/10630/10732
UL http://hdl.handle.net/10630/10732
LA eng
NO Lab Anim 49 (3S) 2015: 60 (ID:60)
NO Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech. CGL2010-16417, BES-2011-046901, Estancias Breves para FPI (2012, 2013), FEDER funds.
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 4 mar 2026