RT Conference Proceedings
T1 Allelic variation of MYB10 is the major force controlling natural variation in skin and flesh color in strawberry (Fragaria spp.) fruit
A1 Castillejo, Cristina
A1 Waurich, Veronika
A1 Wagner, Henning
A1 Ramos, Rubén
A1 Oiza, Nicolás
A1 Muñoz, Pilar
A1 Triviño, Juan C
A1 Caruana, Julie
A1 Liu, Zhongchi
A1 Cobo, Nicolás
A1 Hardigan, Michael A
A1 Knapp, Steven J
A1 Vallarino Castro, José G.
A1 Osorio-Algar, Sonia
A1 Martín-Pizarro, Carmen
A1 Posé-Padilla, David
A1 Toivainen, Tuomas
A1 Hytönen, Timo
A1 Oh, Youngjae
A1 Barbey, Christopher R
A1 Whitaker, Vance M
A1 Lee, Seonghee
A1 Olbricht, Klaus
A1 Sánchez-Sevilla, José F
A1 Amaya Saavedra, Iraida
K1 Fresas
AB Fruits of diploid and octoploid strawberry (Fragaria spp.) show substantial natural variation in both skin and flesh color due to distinct anthocyanin accumulation and distribution patterns. Expression of structural genes involved in anthocyanin biosynthesis is controlled by transcription factors such as bHLH, MYB and WD repeat proteins, among which the R2R3 MYB10 is the main activator in strawberry fruit. Here, we show that independent mutations in MYB10 are behind the natural fruit color variation observed in diploid woodland strawberry (F. vesca) and octoploid cultivated strawberry (F. × ananassa). Using a mapping-by sequencing approach, we identified a gypsy-transposon in MYB10 that truncates the protein and knocks out anthocyanin biosynthesis in a white-fruited F. vesca ecotype. Two additional loss-of-function mutations in MYB10 were identified among geographically diverse white-fruited F. vesca ecotypes. Color variation in octoploid Fragaria spp was further investigated by genetic and transcriptomic analyses, which revealed that FaMYB10-2, one of three MYB10 homoeologs identified, is the dominant homoeolog and regulates anthocyanin biosynthesis in developing fruit. Furthermore, independent mutations in MYB10-2 are the underlying cause of natural variation in fruit skin and flesh color in octoploid strawberry. A genome-wide association study in F. × ananassa and analysis in other octoploid populations identified two independent polymorphisms in FaMYB10-2 causing white skin. Flesh pigmentation was associated with the insertion of a CACTA-like transposon (FaEnSpm-2) in the MYB10-2 promoter and with enhanced MYB10 expression. Our findings suggest that cis-regulatory elements provided by FaEnSpm-2 are responsible for ectopic MYB10-2 expression and anthocyanin biosynthesis in strawberry fruit flesh. Our results show that MYB10 exerts a simple genetic control over strawberry skin and flesh color, making it a good target for molecular breeding.
YR 2021
FD 2021-11-26
LK https://hdl.handle.net/10630/23572
UL https://hdl.handle.net/10630/23572
LA eng
NO RFP2015-00011-00-00 and PID2019-111496RR-I00 (Spanish Research Agency and FEDER), PR.AVA.AVA2019.034 (IFAPA, FEDER funds), ERC-2014-StG 638134 (European Research Council), by the European Union’s Horizon 2020 research and innovation programme (GoodBerry; grant agreement number 679303), and the joint research network SPIRED (German Federal Ministry of Education and Research; BMBF, FKZ 031A216A and B)
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 20 ene 2026