RT Conference Proceedings
T1 Novel polyomavirus and papillomavirus detected in gilthead seabream infected by lymphocystis disease virus
A1 Labella Vera, Alejandro Manuel
A1 Carrera, David
A1 López-Bueno, Alberto
A1 Alejo, Alí
A1 Borrego-García, Juan José
A1 Castro-López, María Dolores
K1 Enfermedades por virus
AB Lymphocystis disease virus (LCDV), a member of the genus Lymphocystivirus, familyIridoviridae, is the etiological agent of the lymphocystis disease (LCD), a common pathologythat has been described in more than 150 different fish species worldwide. Direct sequencingof the virome of lymphocystis lesions from affected gilthead seabream (Sparus aurata) wasused to obtain the complete genome sequence of a new LCDV species, named LCDV-Sa, thatis the largest vertebrate iridovirus sequenced to date. This approach allowed us to assemblethe full-length circular genomes of two previously unknown viruses, tentatively identified asmembers of the Polyomaviridae and Papillomaviridae families, and named Sparus auratapolyomavirus 1 (SaPyV1) and Sparus aurata papillomavirus 1 (SaPV1), respectively. SaPyV1genome is a circular 7,299-bp-long DNA with a 52.1% GC content, and contains five nonoverlappingORFs carried on opposite DNA strands in an organization reminiscent to thatfound in polyomaviruses. Phylogenetic analyses based on the amino acid sequence of thelarge T antigen and the VP1 proteins revealed that SaPyV1 clustered with the other currentlyavailable polyomavirus-like full-length genomes obtained from fish. SaPV1 genomecorresponds to a circular 5,748-bp-long DNA and has a GC content of 39.5%. Although thisgenome is smaller than that of previously described papillomaviruses (about 8 kbp), itpresents a typical papillomavirus organization, with seven ORFs carried on the same strand.Similarity searches identified distant orthologues of the early E1 and E2 proteins involved inreplication and transcription, and late structural proteins L1 and L2. Furthermore, one of theothers ORFs encodes a small protein (52 amino acids) that contains both a pRB binding domain(LXCXE) and a C-terminal PDZ class 2 binding motif, with are elements typically present in thelonger E7 and E6 proteins found in most known papillomaviruses. The conserved genomicorganization, the similarity of the main proteins, and the phylogenetical analysis, based on theamino acid sequence of the L1 protein, support that SaPV1 is the first member of thePapillomaviridae family described in fish. Epizootic surveys carried out in gilthead seabreamfarms in the Mediterranean area showed that LCD is frequently associated with theconcurrent appearance of one or both of the new viruses, 98.3% of diseased fish being positivefor SaPyV1 and/or SaPV1. In LCDV-infected asymptomatic fish, SaPyV1 and/or SaPV1 weredetected in 32.1% of the animals analysed.
PB Institute for Veterinary Medical Research, Budapest, Hungary
YR 2017
FD 2017
LK http://hdl.handle.net/10630/13890
UL http://hdl.handle.net/10630/13890
LA eng
NO Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech.
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 19 ene 2026