RT Conference Proceedings
T1 Effect of bone marrow-derived mesenchymal stem cells on congenital hydrocephalus in the hyh mouse
A1 García-Bonilla, María
A1 Shumilov, Kirill
A1 Vitorica Ferrández, Javier
A1 García-Martín, María Luisa
A1 Muñoz, Carmen
A1 Jiménez, Sebastián
A1 Navarro, Victoria
A1 García-Cuervo, Luisa
A1 Cifuentes-Rueda, Manuel
A1 Claros-Gil, Silvia
A1 Andrades-Gómez, José Antonio
A1 Guitérrez, Antonia
A1 Páez-González, Patricia
A1 Jiménez-Lara, Antonio Jesús
K1 Hidrocefalia
K1 Células madre
AB BackgroundBone marrow-derived mesenchymal stem cells (BM-MSC) are considered as a potential therapeutic tool for neurodegenerative diseases due to their ability for migrating into damaged tissue. Additionally, these cells have been proven to produce neuroprotective factors when they are transplanted into damaged tissue.In this research we uncover a neuroprotective role of BM-MSC on congenital hydrocephalus and we study the molecular mechanisms behind it. Materials and methodsFluorescent BM-MSC were analyzed by flow-cytometry and multilineage cell differentiation. They were brain-ventricle injected into hyh hydrocephalic mice. Wild-type and saline-injected hyh mice were used as controls. Inmunohistochemical analyses were performed in fixed brain sections. Inflammatory reaction and neuroprotective factors were studied using quantitative RT-PCR. Metabolites and osmolytes related to brain damage were studied by High Resolution Magic Angle Spinning spectroscopy (HRMAS). RT-PCR and HRMAS were performed in fresh tissue.ResultsIntegrated BM-MSC were identified inside the periventricular astrocyte reaction. IL-1alpha/beta and IL6 levels indicated the absence of inflammatory response in the transplanted tissue after BM-MSC integration. BM-MSC were found producing neuroprotective factors, including GDNF, NGF, BDNF and VEGF. Reduction of osmolytes and neural/glial-function related metabolites levels were detected in hyh mice after BM-MSC injection. These levels mimicked the wild-type situation and indicate partial recovery.ConclusionsThe BM-MSC can play a neuroprotective effect on congenital hydrocephalus in the hyh mouse by the production of neuroprotective factors and the reduction of osmolytes and metabolites related to tissue damage.
YR 2017
FD 2017-06-28
LK http://hdl.handle.net/10630/14062
UL http://hdl.handle.net/10630/14062
LA eng
NO Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech.
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 20 ene 2026