RT Book, Section
T1 Generating chromosome-located transcriptional fusions to fluorescent proteins for single-cell gene expression analysis in Pseudomonas syringae.
A1 Rufián Plaza, José Sebastián
A1 López-Márquez, Diego
A1 López-Pagán, Nieves
A1 Grant, Murray
A1 Ruiz-Albert, Javier
A1 Beuzón-López, Carmen del Rosario
A2 Medina, Carlos
A2 López Baena, Francisco Javier
K1 Genética bacteriana
K1 Fenotipo
K1 Microscopía de fluorescencia
AB The last decade has seen significant effort directed toward the role of phenotypic heterogeneity in bacterialadaptation. Phenotypic heterogeneity usually refers to phenotypic diversity that takes place throughnongenetic means, independently of environmental induced variation. Recent findings are changing howmicrobiologists analyze bacterial behavior, with a shift from traditional assays averaging large populations tosingle-cell analysis focusing on bacterial individual behavior. Fluorescence-based methods are often used toanalyze single-cell gene expression by flow cytometry, fluorescence microscopy and/or microfluidics.Moreover, fluorescence reporters can also be used to establish where and when are the genes of interestexpressed. In this chapter, we use the model bacterial plant pathogen Pseudomonas syringae to illustrate amethod to generate chromosome-located transcriptional gene fusions to fluorescent reporter genes,without affecting the function of the gene of interest.
PB Springer Nature
SN 978-1-4939-7603-4
YR 2018
FD 2018-01-01
LK https://hdl.handle.net/10630/45835
UL https://hdl.handle.net/10630/45835
LA eng
NO Rufián, J.S., López-Márquez, D., López-Pagán, N., Grant, M., Ruiz-Albert, J., Beuzón, C.R. (2018). Generating Chromosome-Located Transcriptional Fusions to Fluorescent Proteins for Single-Cell Gene Expression Analysis in Pseudomonas syringae . In: Medina, C., López-Baena, F. (eds) Host-Pathogen Interactions. Methods in Molecular Biology, vol 1734
NO https://www.springernature.com/gp/open-science/policies/book-policies
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 3 mar 2026