RT Journal Article
T1 Tripodal penta(p-phenylene) for the biofunctionalization of alkynyl-modified silicon surfaces.
A1 Sánchez-Molina, María
A1 Díaz-Morilla, Amelia
A1 Valpuesta-Fernández, María
A1 Contreras-Cáceres, Rafael
A1 López-Romero, Juan Manuel
A1 López-Ramírez, María Rosa
K1 Nanoestructuras
K1 Silicio
AB Here we report the optimization on the covalent grafting methodology of a tripod-shaped penta(p-phenylene), 1, on alkynyl-terminated silicon surfaces, and the incorporation of an active theophylline derivative, 2, for the specific immobilization of proteins. The tripodal molecule presents azide-terminal groups to be attached onto a silicon surface containing an alkynyl monolayer. Initially, compound 1 has been covalently incorporated on alkynyl-terminated Si wafers, by the copper catalyzed alkyne-azide 1,3-dipolar cycloaddition (CuAAC, a click reaction). The tripod density on the silicon surface is tuned by performing the CuAAC reaction at different concentrations of 1, as well as under different experimental conditions (T, base, copper source, shaking). Then, tripod 1-modified surface has also been biofunctionalized with 2. The effective preparation of this silicon-modified surface allowed us to study the streptavidin immobilization on the surface. Characterization of the different surfaces has been carried out by X-Ray Photoelectron Spectroscopy (XPS), Atomic Force Microscopy (AFM) and Bright-Field Optical Transmission Microscopy (Confocal) techniques. We also include density functional theory (DFT) analysis of the organic structures to confirm the height-profile and the tripod-surface relative configuration extracted from AFM images.
PB Elsevier
YR 2018
FD 2018-03-22
LK https://hdl.handle.net/10630/29340
UL https://hdl.handle.net/10630/29340
LA eng
NO Sánchez-Molina, M., Díaz, A., Valpuesta, M., Contreras-Cáceres, R., López-Romero, J. M., & López-Ramírez, M. R. (2018). Tripodal penta(p-phenylene) for the biofunctionalization of alkynyl-modified silicon surfaces. Applied Surface Science, 445, 175–185.
NO The authors wish to thank the financial support to the Spanish Ministerio de Economía y Competitividad, Projects CTQ13-48418-P and CTQ16-76311-R, FEDER funds
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 21 ene 2026