RT Conference Proceedings
T1 Identification of genes responsible of the synthesis of the volatile Methyl Ketone in Woodland Strawberry
A1 Urrutia, María
A1 Meco, Victoriano
A1 Rambla, José L
A1 Toivainen, Tuomas
A1 Pillet, Jeremy
A1 Trapero Mozos, Almudena
A1 Martín-Pizarro, Carmen
A1 Salas, Joaquín
A1 Granell, Antonio
A1 Hytönen, Timo
A1 Posé-Padilla, David
K1 Fresas
AB Woodland strawberry (Fragaria vesca, 2x) is the diploid closest ancestor of the cultivated strawberry (F. x ananassa, 8x) and the model species for genetic studies in the Fragaria genus. It is naturally distributed across Europe and it is appreciated for its delicate aroma and flavor.Methyl ketones are compounds with demonstrated insect repellent effects (Antonious et al., 2003). Its synthesis, reported to take place in glandular trichomes of wild tomato (Solanum habrochaites), is mediated by a thioesterase (ShMKS2) and a decarboxylase (ShMKS1) (Fridman et al., 2005; Ben-Israel et al., 2009; Yu et al., 2010).Through genome wide association analysis (GWAS) in a natural collection of European woodland strawberry (199 accessions), we identified a series of polymorphisms linked to the accumulation of C7, C9 and C11 methyl ketones (2-heptanone, 2-nonanone, 2-undecanone), their respective secondary alcohols (2-heptanol, 2-nonanol, 2-undecanol) and their precursors (methyl octanoate,methyl decanoate, methyl dodecanoate). In addition, the candidate region presented three different haplotypes with different patterns of methyl ketones accumulation, suggesting differences in enzymatic activity and/or substrateaffinity.The candidate region includes two thioesterases homologues to ShMKS2, FvMKS2A, FvMKS2B. Functional validation of both genes has revealed methyl ketones synthesis ability in transient expression in Nicotiana benthamiana and E. coli. The specificity of these candidate genes is currently under study though several approaches, including transient expression in different hosts andenzymatic function characterization.
YR 2021
FD 2021-11-26
LK https://hdl.handle.net/10630/23574
UL https://hdl.handle.net/10630/23574
LA eng
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 25 ene 2026