RT Conference Proceedings
T1 Contribution of the metabolism of phenolic compounds to the virulence of Pseudomonas savastanoi in woody hosts
A1 Caballo-Ponce, Eloy
A1 Wittich, Regina Michaela
A1 van Dillewijn, Pieter
A1 Ramos-Rodríguez, Cayo Juan
K1 Pseudomonas
AB Most strains of the Pseudomonas syringae complex isolated from the trunks of woodyplants and belonging to phylogroups (PGs) 1 and 3 share a genomic region, named WHOP(from woody host and Pseudomonas), which is partially conserved in some PG2 strains.Annotation of the 14 ORFs encoded within this region, which is absent in the genomes of P.syringae strains infecting herbaceous hosts, yielded functions possibly involved in themetabolism of phenolic compounds. The genetic organization of this region was analyzed byRT-PCR in Pseudomonas savastanoi pv. savastanoi (Psv), the causative agent of olive knotdisease. The WHOP region is organized in four operons (antABC, catBCA, ipoABC and dhoAB)and three genes transcribed independently (antR, benR and PSA3335_3206). HPLC analysesconfirmed that the antABC and catBCA operons mediate the catabolism of anthranilate andcatechol, respectively, through the β-ketoadipate pathway. In addition, oxygenase activityon aromatic compounds, tested as the conversion of indole into indigo, was assigned to theipoABC operon. Pathogenicity tests revealed that deletion of antABC, catBCA or ipoABC inPsv caused reduced symptoms in woody olive plants, while such phenotype was notobserved in young micropropagated (non-woody) plants. Similarly, the catBCA and dhoABoperons and the PSA3335_3206 gene (encoding a putative aerotaxis receptor) are alsorequired for full bacterial fitness exclusively in woody olive plants. At present, we areaddressing the role in virulence of the orphan benR gene encoded in the WHOP region.
YR 2017
FD 2017-09-26
LK http://hdl.handle.net/10630/14539
UL http://hdl.handle.net/10630/14539
LA eng
NO Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech.
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 20 ene 2026