RT Journal Article
T1 Potential protective role of reactive astrocytes in the periventricular parenchyma in congenital hydrocephalus
A1 García-Bonilla, María
A1 Shumilov, Kirill
A1 Peñalver, Ana
A1 Castilla, Laura
A1 Alonso-Carrión, Francisco José
A1 Márquez-Gómez, Javier
A1 García-Martín, María Luisa
A1 Muñoz, Carmen
A1 Gutiérrez-Pérez, Antonia
A1 Jiménez-Lara, Antonio Jesús
K1 Hidrocefalia
AB BackgroundCerebrospinal fluid accumulation in hydrocephalus produces an elevation of intraventricular pressure with pathological consequences on the periventricular brain parenchyma including ischemia, oedema, oxidative stress, and accumulation of metabolic waste products. Here we studied in the hyh mouse, an animal model of congenital hydrocephalus, the role of reactive astrocytes in this clinical degenerative condition.Materials and MethodsWild type and hydrocephalic hyh mice at 30 days of postnatal age were used. Three metabolites related to the oxidative and neurotoxic conditions were analysed in ex vivo samples (glutathione, glutamine and taurine) using High Resolution Magic Angle Spinning (HR-MAS). Glutathione synthetase and peroxidase, glutamine synthetase, kidney-type glutaminase (KGA), and taurine/taurine transporter were immunolocated in brain sections. ResultsLevels of the metabolites were remarkably higher in hydrocephalic conditions. Glutathione peroxidase and synthetase were both detected in the periventricular reactive astrocytes and neurons. Taurine was mostly found free in the periventricular parenchyma and in the reactive astrocytes, and the taurine transporter was mainly present in the neurons located in such regions. Glutamine synthetase was found in reactive astrocytes. Glutaminase was also detected in the reactive astrocytes and in periventricular neurons. These results suggest a possible protective response of reactive astrocytes against oxidative stress and neurotoxic conditions.ConclusionsAstrocyte reaction seems to trigger an anti-oxidative and anti-neurotoxic response in order to ameliorate pathological damage in periventricular areas of the hydrocephalic mice.
YR 2016
FD 2016-07-11
LK http://hdl.handle.net/10630/11797
UL http://hdl.handle.net/10630/11797
LA eng
NO Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech. PI15-00619 to AJJ.
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 25 abr 2026