RT Journal Article
T1 Analysis of Menstrual Blood Stromal Cells Reveals SOX15 Triggers Oocyte-Based Human Cell Reprogramming
A1 López-Caraballo, Lidia
A1 Martorell-Marugán, Jordi
A1 Carmona-Sáez, Pedro
A1 González-Muñoz, María Elena
K1 Ingeniería genética
K1 Menstruación - Aspectos genéticos
K1 Ovulos
AB Cell reprogramming has revolutionized cell and regenerative biology field. However, human iPS derivation remains inefficient and variable. A better knowledge of molecular processes and the rationale underlying the importance of somatic cell origin is crucial to uncover reprogramming mechanisms. Here, we analyze the molecular profile of different human somatic cell types. We show menstrual blood-derived stromal cells (MnSCs) have a distinct, reprogramming prone, profile, and we identify SOX15 from their oocyte-related signature as a prominent responsible candidate. SOX15 orchestrates an efficient oocyte-based reprogramming combination when overexpressed with the also oocyte-enriched histone chaperone ASF1A and OCT4 and, through specific mechanism, generates iPSCs with distinguishable pluripotent state that further present higher differentiation capacity than canonical iPSCs. Our work supports the presence of different pluripotency states in reprogramming and the importance of using metaphase-II oocyte and MnSCs information to provide alternative reprogramming combinations and, importantly, to improve and understand pluripotency acquisition.
PB CellPress
YR 2020
FD 2020
LK https://hdl.handle.net/10630/38815
UL https://hdl.handle.net/10630/38815
LA eng
NO Analysis of Menstrual Blood Stromal Cells Reveals SOX15 Triggers Oocyte-Based Human Cell Reprogramming     Lopez-Caraballo, Lidia et al.     iScience, Volume 23, Issue 8, 101376
NO We acknowledge the assistance and support of Laboratory for Cell Reprogramming and BIONAND students, colleagues, and collaborators. We thank members of the LARCEL laboratory and Prof. J.B. Cibelli (Michigan State University) for comments, discussion, and support; Dr. Ariane Wittgreen for intellectual input and discussion; and Biobanco del Sistema Sanitario Público de Andalucía for karyotyping and teratoma assay service. The authors thankfully acknowledge the computer resources (IPA software) provided by the PAB (Andalusian Bioinformatics Platform) center located at the University of Malaga (www.scbi.uma.es). This work was funded by Ministerio de Economía y Competitividad Gobierno de España (MINECO-SAF2015-66105-R and RYC-2014-15410) and Fundación Progreso y Salud.
NO We acknowledge the assistance and support of Laboratory for Cell Reprogramming and BIONAND students, colleagues, and collaborators. We thank members of the LARCEL laboratory and Prof. J.B. Cibelli (Michigan State University) for comments, discussion, and support; Dr. Ariane Wittgreen for intellectual input and discussion; and Biobanco del Sistema Sanitario Público de Andalucía for karyotyping and teratoma assay service. The authors thankfully acknowledge the computer resources (IPA software) provided by the PAB (Andalusian Bioinformatics Platform) center located at the University of Malaga (www.scbi.uma.es). This work was funded by Ministerio de Economía y Competitividad Gobierno de España (MINECO-SAF2015-66105-R and RYC-2014-15410) and Fundación Progreso y Salud.
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 21 ene 2026