RT Journal Article
T1 Nuclear Translocation of Glutaminase GLS2 in Human Cancer Cells Associates with Proliferation Arrest and Differentiation
A1 López de la Oliva, Amada
A1 Campos-Sandoval, José A.
A1 Gómez-García, María Carmen
A1 Cardona, Carolina
A1 Martín-Rufián, Mercedes
A1 Sialana, Fernando J.
A1 Castilla, Laura
A1 Bae, Narkhyun
A1 Lobo, Carolina
A1 Peñalver, Ana
A1 García-Frutos, Marina
A1 Carro, David
A1 Enrique, Victoria
A1 Paz-Gutiérrez, José Carlos
A1 Mirmira, Raghavendra G.
A1 Gutiérrez, Antonia
A1 Alonso-Carrión, Francisco José
A1 Segura-Checa, Juan Antonio
A1 Mates-Sánchez, José Manuel
A1 Lubec, Gert
A1 Márquez-Gómez, Javier
K1 Glutaminasa
AB Glutaminase (GA) catalyzes the first step in mitochondrial glutaminolysis playing a key role in cancermetabolic reprogramming. Humans express two types of GA isoforms: GLS and GLS2. GLS isozymeshave been consistently related to cell proliferation, but the role of GLS2 in cancer remains poorlyunderstood. GLS2 is repressed in many tumor cells and a better understanding of its function intumorigenesis may further the development of new therapeutic approaches. We analyzed GLS2expression in HCC, GBM and neuroblastoma cells, as well as in monkey COS-7 cells. We studied GLS2expression after induction of differentiation with phorbol ester (PMA) and transduction with the fulllengthcDNA of GLS2. In parallel, we investigated cell cycle progression and levels of p53, p21 and c-Mycproteins. Using the baculovirus system, human GLS2 protein was overexpressed, purified and analyzedfor posttranslational modifications employing a proteomics LC-MS/MS platform. We have demonstrateda dual targeting of GLS2 in human cancer cells.
PB Nature Portfolio
YR 2020
FD 2020-02-10
LK https://hdl.handle.net/10630/32836
UL https://hdl.handle.net/10630/32836
LA eng
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 21 ene 2026