RT Journal Article
T1 Metabolic endotoxemia promotes adipose dysfunction and inflammation in human obesity
A1 Clemente-Postigo, María Mercedes
A1 Oliva Olivera, Wilfredo
A1 Coín-Aragüez, Leticia I.
A1 Ramos-Molina, Bruno
A1 Giráldez-Pérez, Rosa María
A1 Lhamyani, Said
A1 Alcaide Torres, Juan
A1 Pérez-Martínez, Pablo
A1 El Bekay Rizky, Rajaa
A1 Cardona-Díaz, Fernando
A1 Tinahones-Madueño, Francisco José
K1 Obesidad
K1 Tejido adiposo
AB Impaired adipose tissue (AT) lipid handling and inflammation is associated with obesity-related metabolic diseases. Circulating lipopolysaccharides (LPSs) from gut microbiota (metabolic endotoxemia), proposed as a triggering factor for the low-grade inflammation in obesity, might also be responsible for AT dysfunction. Nevertheless, this hypothesis has not been explored in human obesity. To analyze the relationship between metabolic endotoxemia and AT markers for lipogenesis, lipid handling, and inflammation in human obesity, 33 patients with obesity scheduled for surgery were recruited and classified according to their LPS levels. Visceral and subcutaneous AT gene and protein expression were analyzed and adipocyte and AT in vitro assays performed. Subjects with obesity with a high degree of metabolic endotoxemia had lower expression of key genes for AT function and lipogenesis (SREBP1, FABP4, FASN, and LEP) but higher expression of inflammatory genes in visceral and subcutaneous AT than subjects with low LPS levels. In vitro experiments corroborated that LPS are responsible for adipocyte and AT inflammation and downregulation of PPARG, SCD, FABP4, and LEP expression and LEP secretion. Thus, metabolic endotoxemia influences AT physiology in human obesity by decreasing the expression of factors involved in AT lipid handling and function as well as by increasing inflammation.
PB American Physiological Society
YR 2019
FD 2019
LK https://hdl.handle.net/10630/34041
UL https://hdl.handle.net/10630/34041
LA eng
NO Mercedes Clemente-Postigo, Wilfredo Oliva-Olivera, Leticia Coin-Aragüez, Bruno Ramos-Molina, Rosa María Giraldez-Perez, Said Lhamyani, Juan Alcaide-Torres, Pablo Perez-Martinez, Rajaa El Bekay, Fernando Cardona and Francisco J. Tinahones.  Metabolic endotoxemia promotes adipose dysfunction and inflammation in human obesity. American Journal of Physiology-Endocrinology and Metabolism, vol 316, n 2, pp 319-332, 2019. doi:10.1152/ajpendo.00277.2018 PMID: 30422702
NO M. Clemente-Postigo was a recipient of an Formación de Profesorado Universitario (FPU) grant (AP2009-4537) from the Ministry of Education (Madrid, Spain), B. Ramos-Molina was supported by the Sara Borrell program (CD16/00034) from the Instituto de Salud Carlos III (ISCIII; Madrid, Spain), and F. Cardona and R. El Bekay were supported by the Nicolas Monardes program (C-0032-2016 and C-0030-2016) from the Consejería de Salud (Junta de Andalucía, Spain) and cofunded by the Fondo Europeo de Desarrollo Regional (FEDER). This study was supported by Centro de Investigación Biomédica En Red (CIBER, CB06/03/0018) of the ISCIII, Madrid (Spain); P11-CTS-08181 from the Consejería de Economía, Innovación, Ciencia y Empleo (Junta de Andalucía); and PI14/00082, PI15/01114, and PIE14/00031 from the ISCIII; and cofunded by the Fondo Europeo de Desarrollo Regional (FEDER).
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 4 mar 2026