RT Journal Article
T1 Arabidopsis NahG Plants as a Suitable and Efficient System for Transient Expression using Agrobacterium tumefaciens
A1 Rosas-Díaz, Tábata Victoria
A1 Cana Quijada, José Francisco
A1 Amorim-Silva, Vitor
A1 Botella-Mesa, Miguel Ángel
A1 Lozano Durán, Rosa
A1 Bejarano, Eduardo R.
K1 Plantas - Investigación
AB aken together, our results show that expression of the NahG transgene in Arabidopsis dramatically enhances the efficiency of Agrobacterium-mediated transformation in rosette leaves, enabling the routine use of this technique in transient assays. This method can be successfully applied in SA-depleted Arabidopsis plants for transient expression-based functional assays routinely done in N. benthamiana, which would facilitate the use of the plethora of tools and knowledge generated in Arabidopsis. The use of this assay does not require complex inoculation media, supplements, or specific growth conditions, and can be used with different Agrobacterium strains. A high level of expression has also been previously achieved in Arabidopsis transgenic plants containing an inducible cassette to express the bacterial effector AvrPto from a DEX-inducible promoter (Tsuda et al., 2012). However, the assay reported here overcomes the use of DEX treatment and the putative multi-side effects potentially derived from the expression of a bacterial effector that interferes with multiple receptor-like kinases and whose constitutive expression is most likely detrimental for the plant.
PB Science direct
YR 2017
FD 2017-02
LK https://hdl.handle.net/10630/33200
UL https://hdl.handle.net/10630/33200
LA eng
NO Rosas-Díaz et al., 2017
NO This work was supported by the Spanish Ministerio de Ciencia y Tecnología (AGL2013-48913-C2 and BIO2014-55380R); and the Shanghai Center for Plant Stress Biology, Chinese Academy of Sciences, and the 100 Talent Program from the Chinese Academy of Sciences (RL-D). T.R.-D. is funded by a postdoctoral President's International Fellowship Initiative (PIFI) number 2016PB042 from the Chinese Academy of Sciences.
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 19 ene 2026