RT Journal Article
T1 Inorganic nitrogen form determines nutrient allocation and metabolic responses in maritime pine seedlings.
A1 Ortigosa Peña, Francisco
A1 Valderrama-Martín, José Miguel
A1 Urbano-Gámez, Jose Alberto
A1 García Martín, Maria Luisa
A1 Ávila-Sáez, Concepción
A1 Cánovas-Ramos, Francisco Miguel
A1 Cañas-Pendón, Rafael Antonio
K1 Plantas - Efectos del nitrógeno
K1 Nutrientes de las plantas
K1 Pinos - Plántulas
AB Nitrate and ammonium are the main forms of inorganic nitrogen available to plants. The present study aimed to investigate the metabolic changes caused by ammonium and nitrate nutrition in maritime pine (Pinus pinaster Ait.). Seedlings were grown with five solutions containing different proportions of nitrate and ammonium. Their nitrogen status was characterized through analyses of their biomass, different biochemical and molecular markers as well as a metabolite profile using 1H-NMR. Ammonium-fed seedlings exhibited higher biomass than nitrate-fed-seedlings. Nitrate mainly accumulated in the stem and ammonium in the roots. Needles of ammonium-fed seedlings had higher nitrogen and amino acid contents but lower levels of enzyme activities related to nitrogen metabolism. Higher amounts of soluble sugars and L-arginine were found in the roots of ammonium-fed seedlings. In contrast, L-asparagine accumulated in the roots of nitrate-fed seedlings. The differences in the allocation of nitrate and ammonium may function as metabolic buffers to prevent interference with the metabolism of photosynthetic organs. The metabolite profiles observed in the roots suggest problems with carbon and nitrogen assimilation in nitrate-supplied seedlings. Taken together, this new knowledge contributes not only to a better understanding of nitrogen metabolism but also to improving aspects of applied mineral nutrition for conifers.
PB MDPI
SN 2223-7747
YR 2020
FD 2020-04-09
LK https://hdl.handle.net/10630/37918
UL https://hdl.handle.net/10630/37918
LA eng
NO Ortigosa, F.; Valderrama-Martín, J.M.; Urbano-Gámez, J.A.; García-Martín, M.L.; Ávila, C.; Cánovas, F.M.; Cañas, R.A. Inorganic Nitrogen Form Determines Nutrient Allocation and Metabolic Responses in Maritime Pine Seedlings. Plants 2020, 9, 481. https://doi.org/10.3390/plants9040481
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 3 mar 2026