RT Conference Proceedings
T1 The stimulation of the innate immune system by SJNNV protects juvenile european sea bass (Dicentrarchus labrax) against superinfection with RGNNV
A1 Carballo Perez, Carlos
A1 López-Jimena, Benjamín
A1 Álvarez-Torres, Daniel
A1 García-Rosado, Esther
A1 Borrego-García, Juan José
A1 Alonso-Sánchez, María del Carmen
K1 Peces - Inmunología
AB In this study, viral replication and transcription of innate immunogenes have beendetermined by RT-real time-PCR (RT-qPCR) in the course of an experimentalinfection. Three different experimental conditions were considered: i) RGNNVinoculatedanimals; ii) SJNNV-inoculated animals and iii) animals inoculated withSJNNV and superinfected with RGNNV. Superinfection was performed 24 h after theSJNNV inoculation. Control animals were mocked-injected with L-15 medium.The RGNNV-infected group showed typical symptoms of the disease and displayed76% cumulative mortality at the end of the experiment, whereas the mortality in thesuperinfected group was 4%, and no mortality was recorded in the SJNNV-inoculatedgroup. The analysis of the Mx transcription by RT-qPCR showed a clearly differentialinduction of the sea bass innate immune system by RGNNV and SJNNV, since notranscription was recorded at any time tested (from 0 h to 48 h p.i.) after the RGNNVinoculation, whereas the injection of SJNNV resulted in an important increase of theMx transcription from 24 h p.i. onwards. In the superinfected group the induction ofthe Mx gene transcription follows the same patterns that the ones described for thegroups inoculated with SJNNV and RGNNV separately. These results suggest that theinduction of the IFN mediated system by the previous infection with SJNNV could beresponsible for the decrease in the mortality recorded in the superinfected group,protecting sea bass of the posterior infection with RGNNV.
YR 2013
FD 2013-12-04
LK http://hdl.handle.net/10630/6738
UL http://hdl.handle.net/10630/6738
LA eng
NO Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech.
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 21 ene 2026