RT Book, Section
T1 Generation and use of site-directed chromosomal cyaA’ translational fusions in Salmonella enterica.
A1 Ramos Morales, Francisco
A1 Cardenal Muñoz, Elena
A1 Cordero Alba, Mar
A1 Baisón Olmo, Fernando
K1 Translocación (Genética)
K1 Salmonella - Genética
K1 Bacterias gram negativas
K1 Bacterias - Metabolismo
AB CyaA from Bordetella pertussis is a calmodulin-dependent adenylate cyclase. Fusions to the catalytic domain of CyaA (CyaA’) are useful tools to detect translocation of type III secretion system effectors from Gram-negative pathogens like Salmonella enterica. These fusions are usually generated using plasmids with strong promoters. Here, we describe a protocol to insert the CyaA’-encoding sequence in a specific site in the bacterial chromosome in order to get a monocopy fusion whose expression is driven by the native promoter. We also describe the procedure to detect translocation of a CyaA’ fusion into mammalian cells.
PB Springer
YR 2015
FD 2015
LK https://hdl.handle.net/10630/37565
UL https://hdl.handle.net/10630/37565
LA eng
NO Ramos-Morales F, Cardenal-Muñoz E, Cordero-Alba M, Baisón-Olmo F. Generation and use of site-directed chromosomal cyaA' translational fusions in Salmonella enterica. Methods Mol Biol. 2015;1225:93-104. doi: 10.1007/978-1-4939-1625-2_6. PMID: 25253250.
NO https://www.springernature.com/gp/open-science/policies/book-policies
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 24 ene 2026