RT Journal Article
T1 Integrated single-base resolution maps of transcriptome, sRNAome and methylome of Tomato yellow leaf curl virus (TYLCV) in tomato
A1 Piedra-Aguilera, Álvaro
A1 Jiao, Chen
A1 Pérez-Luna, Ana Isabel
A1 Villanueva, Francisco
A1 Dabad, Marc
A1 Esteve-Codina, Anna
A1 Diaz-Pendón, Juan Antonio
A1 Fei, Zhangjun
A1 Rodríguez-Bejarano, Eduardo
A1 Castillo-Garriga, Araceli
K1 Virus fitopatógenos
K1 Tomates - Enfermedades y plagas
AB Geminiviruses are plant ssDNA viruses that replicate through dsDNA intermediates and formminichromosomes which carry the same epigenetic marks as the host chromatin. During the infection,geminiviruses are targets of the post-transcriptional and transcriptional gene silencing machinery.To obtain insights into the connection between virus-derived small RNAs (vsRNAs), viral genomemethylation and gene expression, we obtained the transcriptome, sRNAome and methylome fromthe geminivirus Tomato yellow leaf curl virus-infected tomato plants. The results showed accumulationof transcripts just at the viral ORFs, while vsRNAs spanned the entire genome, showing a prevalentaccumulation at regions where the viral ORFs overlapped. The viral genome was not homogenouslymethylated showing two highly methylated regions located in the C1 ORF and around the intergenicregion (IR). The compilation of those results showed a partial correlation between vsRNA accumulation,gene expression and DNA methylation. We could distinguish different epigenetic scenarios alongthe viral genome, suggesting that in addition to its function as a plant defence mechanism, DNAmethylation could have a role in viral gene regulation. To our knowledge, this is the first report thatshows integrative single-nucleotide maps of DNA methylation, vsRNA accumulation and geneexpression from a plant virus.
PB Springer Nature
YR 2019
FD 2019-02-27
LK https://hdl.handle.net/10630/34091
UL https://hdl.handle.net/10630/34091
LA spa
NO Piedra-Aguilera, Á., Jiao, C., Luna, A.P. et al. Integrated single-base resolution maps of transcriptome, sRNAome and methylome of Tomato yellow leaf curl virus (TYLCV) in tomato. Sci Rep 9, 2863 (2019). https://doi.org/10.1038/s41598-019-39239-6
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 19 ene 2026