RT Journal Article
T1 Defender or accomplice? Dual roles of plant vesicle trafficking in restricting and enabling geminiviral systemic infection
A1 Cana Quijada, José Francisco
A1 Morales Martínez, Pablo
A1 Rosas-Díaz, Tábata Victoria
A1 Pérez-Sancho, Jessica
A1 Jiménez-Góngora, Tamara
A1 Navarro, José Antonio
A1 Lozano-Durán, Rosa
A1 Castillo-Garriga, Araceli
A1 Pallás, Vicente
A1 Rodríguez-Bejarano, Eduardo
K1 Tomates - Enfermedades y plagas
K1 Virus fitopatógenos
AB The vesicle trafficking system enables multidirectional cargo fluxes between endomembrane compartments. However, vesicle trafficking plays dual roles during pathogen infections. In plants, it mediates autophagic immune responses but can also be hijacked by pathogens to facilitate successful infections.    We demonstrate that vesicle trafficking machinery acts as a double-edged sword during infection by the geminivirus tomato yellow leaf curl Sardinia virus (TYLCSaV) in Nicotiana benthamiana. Virus-induced gene silencing of eight genes encoding key vesicle trafficking regulators revealed contrasting outcomes. Silencing of NbSAR1 and NbAP-1γ significantly increased systemic geminiviral DNA accumulation, whereas silencing of Nbδ-COP, NbARF1 and clathrin genes almost completely abolished infection. Notably, this inhibition is hypothesized to result from direct or indirect impairment in viral movement, as replication remained unaffected by gene silencing.    The observed effects affect other geminiviruses, including tomato yellow leaf curl virus (TYLCV) and beet curly top virus (BCTV), but not unrelated pathogens, such as the RNA potato virus X (PVX) or the plant pathogenic bacterium Pseudomonas syringae.    These findings suggest that while the vacuolar and autophagy branches of the vesicle trafficking system might mediate antiviral autophagic defence responses, the integrity of endocytosis and retrograde transport is essential for systemic geminiviral infection.
PB Wiley
YR 2025
FD 2025
LK https://hdl.handle.net/10630/41028
UL https://hdl.handle.net/10630/41028
LA eng
NO Cana-Quijada, P., Morales-Martínez, P., Rosas-Díaz, T., Pérez-Sancho, J., Jiménez-Góngora, T., Navarro, J.A., Lozano-Durán, R., Castillo, A.G., Pallás, V. and Bejarano, E.R. (2025), Defender or accomplice? Dual roles of plant vesicle trafficking in restricting and enabling geminiviral systemic infection. New Phytol. https://doi.org/10.1111/nph.70707
NO Disponible online 30 de Noviembre 2025
NO Funding for open access charge: Universidad de Málaga / CBUA
NO This work was funded by the Plan Nacional I + D + i, Ministerio de Economía y Competitividad (Agencia Estatal de Investigación), Spain
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 14 abr 2026