RT Conference Proceedings
T1 Identification of candidate genes for methylketones content in a collection of european woodland strawberry
A1 Urrutia, María
A1 Meco, Victoriano
A1 Rambla, José Luis
A1 Toivainen, Tuomas
A1 Pillet, Jeremy
A1 Trapero-Mozos, Almudena
A1 Martín-Pizarro, Carmen
A1 Sánchez-Gómez, Carlos
A1 Salas, Joaquín
A1 Granell, Antonio
A1 Hytönen, Timo
A1 Posé-Padilla, David
K1 Fresas
K1 Olores
AB Woodland strawberry (Fragaria vesca, 2x) is the diploid closest ancestor of the cultivated strawberry (Fragaria xannassa, 8x) and the model species for genetic studies in the Fragaria genus. It is naturally distributed all across Europe and it is appreciated for their delicate aroma and flavor.Methylketones are compounds with demonstrated insect repellent effects. Its synthesis, reported to take place in glandular trichomes of wild tomato (Solanum habrochaites), is mediated by a thioesterase (ShMKS2) and adecarboxylase (ShMKS1) (Ben Israel et al. 2009; Yu et al. 2010).A natural population of 199 accessions of F. vesca that represents the European diversity was resequenced (>1.8M SNPs) and characterized for the accumulation of methylketones and their derived alcohols. By genome wide association analysis (GWAS), we have detected a stable series of polymorphisms associated to the accumulation of C7, C9 and C11 methylketones and their respective alcohols. Three different haplotypes in the candidate region with different patterns of methylketones were found, suggesting differences in enzymatic activity and/or substrate affinity. The candidate region includes two genes annotated as thioesterases that are homolog to ShMKS2, FvMKS2A, FvMKS2B. Functional validation of both genes has revealed methyl ketones synthesis ability in transient expression in Nicotiana benthamiana. The function and specificity of these candidate genes is currently under study through several approaches, including transient expression in different hosts and enzymatic function characterization.
YR 2021
FD 2021-01-22
LK https://hdl.handle.net/10630/20822
UL https://hdl.handle.net/10630/20822
LA eng
NO Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech.
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 20 ene 2026