RT Journal Article
T1 Autophagy is required for gamete differentiation in the moss Physcomitrella patens.
A1 Sanchez-Vera, Victoria
A1 Kenchappa, Chandra Shekar
A1 Landberg, Katarina
A1 Bressendorf, Simon
A1 Schwarzbach, Stefan
A1 Martin, Tom
A1 Mundy, John
A1 Petersen, Morten
A1 Thelander, Mattias
A1 Sundberg, Eva
K1 Metabolismo
K1 Células - Diferenciación
K1 Musgos
K1 Gametos
K1 Espermatogénesis
AB Autophagy, a major catabolic process in eukaryotes, was initially related to cell tolerance to nutrient depletion. In plants autophagy has also been widely related to tolerance to biotic and abiotic stresses (through the induction or repression of programmed cell death, PCD) as well as to promotion of developmentally regulated PCD, starch degradation or caloric restriction important for life span. Much less is known regarding its role in plant cell differentiation. Here we show that macroautophagy, the autophagy pathway driven by engulfment of cytoplasmic components by autophagosomes and its subsequent degradation in vacuoles, is highly active during germ cell differentiation in the early diverging land plant Physcomitrella patens. Our data provide evidence that suppression of ATG5-mediated autophagy results in reduced density of the egg cell-mediated mucilage that surrounds the mature egg, pointing toward a potential role of autophagy in extracellular mucilage formation. In addition, we foundthat ATG5- and ATG7-mediated autophagy is essential for the differentiation and cytoplasmic reduction of the flagellated motile sperm and hence for sperm fertility. The similarities between the need of macroautophagy for sperm differentiation in moss and mouse are striking, strongly pointing toward an ancestral function of autophagy not only as a protector against nutrient stress, but also in gamete differentiation.
PB Autophagy / Landes Biosciences
YR 2017
FD 2017-09-25
LK https://hdl.handle.net/10630/34366
UL https://hdl.handle.net/10630/34366
LA eng
NO Sanchez-Vera, V., Kenchappa, C. S., Landberg, K., Bressendorff, S., Schwarzbach, S., Martin, T., … Sundberg, E. (2017). Autophagy is required for gamete differentiation in the moss Physcomitrella patens. Autophagy, 13(11), 1939–1951.
NO This work was supported by grants to ES from the Knut and Alice Wallenberg Foundation (# 2012.0087) and the Swedish Research Council (# 621–2014–4941), to MT from the Carl Trygger Foundation (12:493, 14:481), the Nilsson-Ehle Endowments and the Olle Engkvist Foundation and to JM from the Danish Research Council on Nature and the Universe (#1323–00267) and the Indo-Danish Research Program (2 research-exchange grants).
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 20 ene 2026