RT Journal Article
T1 The Mangotoxin Biosynthetic Operon (mbo) Is Specifically Distributed within Pseudomonas syringae Genomospecies 1 and Was Acquired Only Once during Evolution.
A1 Carrión Bravo, Víctor José
A1 Gutiérrez Barranquero, José Antonio
A1 Arrebola-Díez, Eva María
A1 Bardaji, Leire
A1 Codina, Juan C.
A1 De-Vicente-Moreno, Antonio
A1 Cazorla-López, Francisco Manuel
A1 Murillo, Jesús
K1 Fitotoxinas
K1 Antimetabolitos
K1 Bacterias fitopatógenas
K1 Operones
AB Mangotoxin production was first described in Pseudomonas syringae pv. syringae strains. A phenotypic characterization of 94 P.syringae strains was carried out to determine the genetic evolution of the mangotoxin biosynthetic operon (mbo). We designed aPCR primer pair specific for the mbo operon to examine its distribution within the P. syringae complex. These primers amplifieda 692-bp DNA fragment from 52 mangotoxin-producing strains and from 7 non-mangotoxin-producing strains that harbor thembo operon, whereas 35 non-mangotoxin-producing strains did not yield any amplification. This, together with the analysis ofdraft genomes, allowed the identification of the mbo operon in five pathovars (pathovars aptata, avellanae, japonica, pisi, andsyringae), all of which belong to genomospecies 1, suggesting a limited distribution of the mbo genes in the P. syringae complex.Phylogenetic analyses using partial sequences from housekeeping genes differentiated three groups within genomospecies 1. Allof the strains containing the mbo operon clustered in groups I and II, whereas those lacking the operon clustered in group III;however, the relative branching order of these three groups is dependent on the genes used to construct the phylogeny. The mbooperon maintains synteny and is inserted in the same genomic location, with high sequence conservation around the insertionpoint, for all the strains in groups I and II. These data support the idea that the mbo operon was acquired horizontally and onlyonce by the ancestor of groups I and II from genomospecies 1 within the P. syringae complex.
PB ASM Journals
YR 2013
FD 2013-01-14
LK https://hdl.handle.net/10630/31138
UL https://hdl.handle.net/10630/31138
LA eng
NO Carrión VJ, Gutiérrez-Barranquero JA, Arrebola E, Bardaji L, Codina JC, de Vicente A, Cazorla FM, Murillo J. The mangotoxin biosynthetic operon (mbo) is specifically distributed within Pseudomonas syringae genomospecies 1 and was acquired only once during evolution. Appl Environ Microbiol. 2013 Feb;79(3):756-67.
NO Política de acceso abierto tomada de: https://v2.sherpa.ac.uk/id/publication/6214
NO This work was supported by grants from the regional government of Andalucía (Spain), grants from CICE-Junta de Andalucía, Ayudas Grupo PAIDI AGR-169, and Proyecto de Excelencia (P07-AGR-02471) and Plan Nacional I D I grant AGL2008-55311-CO2-01 (Ministerio de Ciencia e Innovación), cofinanced by FEDER (European Union). Plan Propio of the University of Málaga funded a stay by V.J.C. at the Universidad Pública de Navarra, Spain. V.J.C. was supported with a fellowship from the Junta de Andalucía, Spain, and E.A. was supported with a JAEDoc grant from the CSIC, which was cofinanced by ESF.
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 20 ene 2026