RT Journal Article
T1 Deciphering the regulatory network of the NAC transcription factor FvRIF, a key regulator of strawberry (Fragaria vesca) fruit ripening
A1 Li, Xiaojinng
A1 Martín-Pizarro, Carmen
A1 Zhou, Leilei
A1 Hou, Bingzhu
A1 Wang, Yuying
A1 Shen, Yuanyue
A1 Li, Bingbing
A1 Posé-Padilla, David
A1 Qin, Guozheng
K1 Fresas
AB The NAC transcription factor ripening inducing factor (RIF) was previously reported to be necessary for the ripening of octoploid strawberry (Fragaria × ananassa) fruit, but the mechanistic basis of RIF-mediated transcriptional regulation and how RIF activity is modulated remains elusive. Here, we show that FvRIF in diploid strawberry, Fragaria vesca, is a key regulator in the control of fruit ripening and that knockout mutations of FvRIF result in a complete block of fruit ripening. DNA affinity purification sequencing coupled with transcriptome deep sequencing suggests that 2,080 genes are direct targets of FvRIF-mediated regulation, including those related to various aspects of fruit ripening. We provide evidence that FvRIF modulates anthocyanin biosynthesis and fruit softening by directly regulating the related core genes. Moreover, we demonstrate that FvRIF interacts with and serves as a substrate of MAP kinase 6 (FvMAPK6), which regulates the transcriptional activation function of FvRIF by phosphorylating FvRIF at Thr-310. Our findings uncover the FvRIF-mediated transcriptional regulatory network in controlling strawberry fruit ripening and highlight the physiological significance of phosphorylation modification on FvRIF activity in ripening.
PB Oxford University Press
YR 2023
FD 2023-07-28
LK https://hdl.handle.net/10630/35673
UL https://hdl.handle.net/10630/35673
LA eng
NO Xiaojing Li, Carmen Martín-Pizarro, Leilei Zhou, Bingzhu Hou, Yuying Wang, Yuanyue Shen, Bingbing Li, David Posé, Guozheng Qin, Deciphering the regulatory network of the NAC transcription factor FvRIF, a key regulator of strawberry (Fragaria vesca) fruit ripening, The Plant Cell, Volume 35, Issue 11, November 2023, Pages 4020–4045, https://doi.org/10.1093/plcell/koad210
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 19 ene 2026