RT Conference Proceedings
T1 Susceptibility of juvenile european sea bass (dicentrarchus labrax) to different viral nervousnecrosis virus (VNNV) isolates
A1 López-Jimena, Benjamín
A1 García-Rosado, Esther
A1 Souto-Pereira, Sandra
A1 Infante, Carlos
A1 Borrego-García, Juan José
A1 Alonso-Sánchez, María del Carmen
K1 Virología - Investigación
AB In this study, the susceptibility of 5-g juvenile European sea basswas evaluated by intramuscular injection (105 TCID50/g) using isolates belongingto the RGNNV and SJNNV genotypes as well as a reassortant isolate (RGNNVRNA1/SJNNV RNA2) obtained from Senegalese sole (Solea senegalensis).In these experimental infections, the cumulative mortality was determined.Furthermore, quantification of viral genome, by absolute real time PCR, andinfective viral particles, by virus titration, was performed from brains of dead andsurvivor fish (30 days post-inoculation). In addition, anti-VNNV antibodies insera from survivor animals were determined by indirect ELISA. Typicalsymptoms of VNN and mortality were only recorded in fish inoculated with theRGNNV (47% cumulative mortality) and the reassortant (33%) isolates.However, high levels of viral genome and infective viral particles were recordedin brain of survivor fish inoculated with the SJNNV isolate, although did notcause mortality or clinical signs. Specific antibody response, measured by indirectELISA, was only observed in the VNNV-inoculated groups, with titres of 1/1024,1/4096 and 1/8192 for RGNNV, SJNNV and reassortant inoculated animals,respectively.
PB EAFP
YR 2013
FD 2013-09-02
LK http://hdl.handle.net/10630/6741
UL http://hdl.handle.net/10630/6741
LA eng
NO UNIVERSIDAD DE MÁLAGA. CAMPUS DE EXCELENCIA INTERNACIONAL ANDALUCÍA TECH
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 3 mar 2026