RT Journal Article
T1 The mbo operon is specific and essential for biosynthesis of mangotoxin in Pseudomonas syringae.
A1 Carrión Bravo, Víctor José
A1 Arrebola-Díez, Eva María
A1 Cazorla-López, Francisco Manuel
A1 Murillo, Jesús
A1 De-Vicente-Moreno, Antonio
K1 Pseudomonas syringae
K1 Bacterias fitopatógenas
K1 Antimetabolitos
K1 Fitotoxinas
K1 Rizobacterias
AB Mangotoxin is an antimetabolite toxin produced by certain Pseudomonas syringae pv. syringae strains. This toxin is anoligopeptide that inhibits ornithine N-acetyl transferase, a key enzyme in the biosynthesis of ornithine and arginine.Previous studies have reported the involvement of the putative nonribosomal peptide synthetase MgoA in virulence andmangotoxin production. In this study, we analyse a new chromosomal region of P. syringae pv. syringae UMAF0158, whichcontains six coding sequences arranged as an operon (mbo operon). The mbo operon was detected in only mangotoxinproducingstrains, and it was shown to be essential for the biosynthesis of this toxin. Mutants in each of the six ORFs of thembo operon were partially or completely impaired in the production of the toxin. In addition, Pseudomonas spp.mangotoxin non-producer strains transformed with the mbo operon gained the ability to produce mangotoxin, indicatingthat this operon contains all the genetic information necessary for mangotoxin biosynthesis. The generation of a singletranscript for the mbo operon was confirmed and supported by the allocation of a unique promoter and Rho-independentterminator. The phylogenetic analysis of the P. syringae strains harbouring the mbo operon revealed that these strainsclustered together.
PB PLOS
YR 2012
FD 2012-05-17
LK https://hdl.handle.net/10630/31131
UL https://hdl.handle.net/10630/31131
LA eng
NO Carrión VJ, Arrebola E, Cazorla FM, Murillo J, de Vicente A (2012) The mbo Operon Is Specific and Essential for Biosynthesis of Mangotoxin in Pseudomonas syringae. PLoS ONE 7(5): e36709
NO This work has been supported through grants from the Regional Government of Andalucı´a (Spain), grants from CICE - Junta de Andalucı´a, Ayudas Grupo PAIDI AGR-169 and Proyecto de Excelencia (P07-AGR-23 02471), and co-financed by FEDER (EU). Plan Propio of the University of Ma´laga funded visitation of VJC to the Public University of Navarra, Spain. VJC was granted with a fellowship from Junta de Andalucı´a, Spain. EA was supported through grants from the CSIC programme mode JAEDoc – Junta para la Ampliacio´n de Estudios, and co-financed by ESF. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 20 ene 2026