RT Journal Article
T1 Transcriptional stimulation of rate-limiting components of the autophagic pathway improves plant fitness.
A1 Minina, Elena A.
A1 Moschou, Panagiotis N.
A1 Vetukuri, Ramesh R.
A1 Sánchez-Vera, Victoria
A1 Cardoso, Catarina
A1 Liu, Qinsong
A1 Elander, Pernilla H.
A1 Dalman, Kerstin
A1 Beganovic, Mirela
A1 Lindberg, Jenny
A1 Marmon, Sofia
A1 Shabala, Lana
A1 Suárez-Marín, María Fernanda
A1 Ljung, Karin
A1 Novak, Ondrej
A1 Shabala, Sergei
A1 Stymne, Sten
A1 Hofius, Daniel
A1 Bozhkov, Peter V.
K1 Metabolismo
K1 Biomasa vegetal
K1 Plantas - Efectos del estrés
K1 Plantas - Envejecimiento
AB Autophagy is a major catabolic process whereby autophagosomes deliver cytoplasmic content to the lytic com- partment for recycling. Autophagosome formation requires two ubiquitin-like systems conjugating Atg12 with Atg5, and Atg8 with lipid phosphatidylethanolamine (PE), respectively. Genetic suppression of these systems causes autophagy-deficient phenotypes with reduced fitness and longevity. We show that Atg5 and the E1-like enzyme, Atg7, are rate-limiting components of Atg8–PE conjugation in Arabidopsis. Overexpression of ATG5 or ATG7 stimulates Atg8 lipidation, autophagosome formation, and autophagic flux. It also induces transcriptional changes opposite to those observed in atg5 and atg7 mutants, favoring stress resistance and growth. As a result, ATG5- or ATG7-overexpressing plants exhibit increased resistance to necrotrophic pathogens and oxidative stress, delayed aging and enhanced growth, seed set, and seed oil content. This work provides an experimental paradigm and mechanistic insight into genetic stimulation of autophagy in planta and shows its efficiency for improving plant productivity.
PB Journal of Experimental Botany / Oxford Academic
YR 2018
FD 2018-01-20
LK https://hdl.handle.net/10630/34376
UL https://hdl.handle.net/10630/34376
LA eng
NO Elena A Minina, Panagiotis N Moschou, Ramesh R Vetukuri, Victoria Sanchez-Vera, Catarina Cardoso, Qinsong Liu, Pernilla H Elander, Kerstin Dalman, Mirela Beganovic, Jenny Lindberg Yilmaz, Sofia Marmon, Lana Shabala, Maria F Suarez, Karin Ljung, Ondřej Novák, Sergey Shabala, Sten Stymne, Daniel Hofius, Peter V Bozhkov, Transcriptional stimulation of rate-limiting components of the autophagic pathway improves plant fitness, Journal of Experimental Botany, Volume 69, Issue 6, 5 March 2018, Pages 1415–1432.
NO This work was supported by grants from the Knut and Alice Wallenberg Foundation (to DH and PVB, 19679-006), the Swedish Research Council (to PVB, 20419-000), Pehrssons Fond (to PVB, 16406-000), the Swedish Foundation for Strategic Research (to StSt and PVB, 21236-000), Olle Engkvist Foundation (to PVB, 18510-000), Carl Tryggers Foundation (to EAM, 21196-000) and Trees and Crops for the Future Research Programme (to PVB).
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 19 ene 2026