RT Journal Article
T1 Classification of Isolates from the Pseudomonas fluorescens Complex into Phylogenomic Groups Based in Group-Specific Markers.
A1 Garrido-Sanz, Daniel
A1 Arrebola-Díez, Eva María
A1 Martínez-Granero, Francisco
A1 García-Méndez, Sonia
A1 Muriel, Candela
A1 Blanco-Romero, Esther
A1 Martín, Marta
A1 Rivilla, Rafael
A1 Redondo-Nieto, Miguel
K1 Pseudomonas fluorescens
K1 Bacterias fitopatógenas
K1 Plantas - Microbiología
AB The Pseudomonas fluorescens complex of species includes plant-associated bacteriawith potential biotechnological applications in agriculture and environmental protection. Many of these bacteria can promote plant growth by different means, including modification of plant hormonal balance and biocontrol. The P. fluorescens group is currently divided into eight major subgroups in which these properties and many other ecophysiological traits are phylogenetically distributed. Therefore, a rapid phylogroup assignment for a particular isolate could be useful to simplify the screening of putative inoculants. By using comparative genomics on 71 P. fluorescens genomes, we have identified nine markers which allow classification of any isolate into these eight subgroups, by a presence/absence PCR test. Nine primer pairs were developed for the amplification of these markers. The specificity and sensitivity of these primer pairs were assessed on 28 field isolates, environmental samples from soil and rhizosphere and tested by in silico PCR on 421 genomes. Phylogenomic analysis validated the results: the PCR-based system for classification of P. fluorescens isolates has a 98.34% of accuracy and it could be used as a rapid and simple assay to evaluate the potential of any P. fluorescens complex strain.
PB Frontiers
YR 2017
FD 2017-03-15
LK https://hdl.handle.net/10630/31139
UL https://hdl.handle.net/10630/31139
LA eng
NO Garrido-Sanz D, Arrebola E, Martínez-Granero F, García-Méndez S, Muriel C, Blanco-Romero E, Martín M, Rivilla R, Redondo-Nieto M. Classification of Isolates from the Pseudomonas fluorescens Complex into Phylogenomic Groups Based in Group-Specific Markers. Front Microbiol. 2017 Mar 15;8:413.
NO Research was funded by grant BIO2015-64480R from MINECO/FEDER EU. DGS was granted by FPU fellowship program (FPU14/03965) from Ministerio de Educación, Cultura y Deporte, Spain
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 28 ene 2026