RT Conference Proceedings
T1 Scaffold-mediated Assembly of the Brassinosteroid Signaling Components by TTL proteins
T2 Ensamblaje de componentes de señalización de brasinoesteroides por las proteínas TTL
A1 Amorim-Silva, Vitor
A1 García-Moreno, Álvaro
A1 Lakhssassi, Naoufal
A1 Esteban del Valle, Alicia
A1 Rosado Rey, Abel
A1 Pérez-Sancho, Jessica
A1 Li, Yansha
A1 Castillo-Garriga, Araceli
A1 Pérez-Rodríguez, Josefa
A1 Valpuesta-Fernández, Victoriano
A1 Zipfel, Cyril
A1 Macho, Alberto P.
A1 Botella-Mesa, Miguel Ángel
K1 Hormonas vegetales
AB Brassinosteroids (BRs) is a group of steroidal hormones that play critical roles in multiple aspects of plant growth and development. BR stimulation at the PM receptors initiates a series of phosphorylation events enabling the nuclear accumulation and activity of the key transcription factor BZR1 and BES1. Previous studies have shown that all BR components associate form an interconnected signaling pathway, although it is not known how these proteins are brought together for the prompt signal transduction upon BR perception. Here we report that plant-specific Tetratricopeptide Thioredoxin-Like TTL proteins are positive regulators of BR signaling functioning as a scaffold of the BR pathway in Arabidopsis. TTL3 interacts with most core components involved in transducing BR signaling, BRI1, BSK1, and BIN2 kinases, the BSU1 phosphatase and the transcription factors BZR1 and BES1. Consistent with this role in BR signaling, mutations in TTL1, TTL2, and TT4 genes cause reduced BR responses, defects that are highly enhanced in a triple ttl1/ttl3/ttl4 mutant. We also show that a functional TTL3-green fluorescence protein is mainly localized in the cytoplasm and that BR treatment increases the association to the plasma membrane. We also show that the cytoplasmic/plasma membrane localization of a functional TTL3-green fluorescence protein is dependent on BR. We propose a novel mechanistic model for optimized BR signaling, in which cytoplasmic/nuclear BR components bound to TTL proteins are translocated to the plasma membrane upon BR perception, which in turn allow the assembly of a BR signaling complex with the goal of ensuring TF de-phosphorylation and nuclear accumulation of the transcription factors.
YR 2017
FD 2017-06-23
LK http://hdl.handle.net/10630/13984
UL http://hdl.handle.net/10630/13984
LA spa
NO Es una comunicación a un Congreso Internacional de primeralínea en el campo objeto de la Tesis Doctoral de Álvaro García
NO Universidad de Málaga. Campus de Excelencia Internacional Andalucía Tech. This work was supported by grants from: (1) Ministerio de Ciencia e Innovación AGL2013-48913-C2-2-R and BIO2014-55380-R; (2) Ministerio de Economía, Industria y Competitividad (BES-2015-071256)
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 21 ene 2026