RT Journal Article
T1 Ethylene is involved in strawberry fruit ripening in an organ-specific manner.
A1 Merchante, Catharina
A1 Valpuesta, Victoriano
A1 Vallarino, José G.
A1 Osorio-Algar, Sonia
A1 Aragüez, Irene
A1 Villareal, Natalia
A1 Ariza, Maria T.
A1 Martínez, Gustavo A.
A1 Medina-Escobar, Nieves
A1 Civello, Marcos P.
A1 Fernie, Alisdair R.
A1 Botella-Mesa, Miguel Ángel
K1 Fresas - Maduración
K1 Etileno
K1 Perfil metabólico
AB The fruit of the strawberry F.ananassa has traditionally been classified as non-climacteric because its ripen-ing process is not governed by ethylene. However, previous studies have reported the timely endogenous productionof minor amounts of ethylene by the fruit as well as the differential expression of genes of the ethylene synthesis,reception, and signalling pathways during fruit development. Mining of the F.vesca genome allowed for theidentification of the two main ethylene biosynthetic genes, 1-aminocyclopropane-1-carboxylic acid (ACC) synthaseand ACC oxidase. Their expression pattern during fruit ripening was found to be stage and organ (achene or recepta-cle) specific. Strawberry plants with altered sensitivity to ethylene could be employed to unravel the role of ethylenein the ripening process of the strawberry fruit. To this end, independent lines of transgenic strawberry plants weregenerated that overexpress the Arabidopsis etr1-1 mutant ethylene receptor, which is a dominant negative allele,causing diminished sensitivity to ethylene. Genes involved in ethylene perception as well as in its related downstreamprocesses, such as flavonoid biosynthesis, pectin metabolism, and volatile biosynthesis, were differently expressedin two transgenic tissues, the achene and the receptacle. The different transcriptional responsiveness of the acheneand the receptacle to ethylene was also revealed by the metabolic profiling of the primary metabolites in these twoorgans. The free amino acid content was higher in the transgenic lines compared with the control in the matureachene, while glucose and fructose, and citric and malic acids were at lower levels. In the receptacle, the most con-spicuous change in the transgenic lines was the depletion of the tricarboxylic acid cycle intermediates at the whitestage of development, most probably as a consequence of diminished respiration.
PB Oxford Academic
YR 2013
FD 2013-10-05
LK https://hdl.handle.net/10630/34505
UL https://hdl.handle.net/10630/34505
LA eng
NO Catharina Merchante, José G. Vallarino, Sonia Osorio, Irene Aragüez, Natalia Villarreal, María T. Ariza, Gustavo A. Martínez, Nieves Medina-Escobar, Marcos P. Civello, Alisdair R. Fernie, Miguel A. Botella, Victoriano Valpuesta, Ethylene is involved in strawberry fruit ripening in an organ-specific manner, Journal of Experimental Botany, Volume 64, Issue 14, November 2013, Pages 4421–4439.
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 20 ene 2026