RT Journal Article
T1 miR825-5p targets the TIR-NBS-LRR gene MIST1 and down-regulates basal immunity against Pseudomonas syringae in Arabidopsis
A1 López-Márquez, Diego
A1 Del Espino Pérez, Ángel
A1 López-Pagán, Nieves
A1 Rodrígez-Negrete, Edgar A.
A1 Rubio-Somoza, Ignacio
A1 Ruiz-Albert, Francisco Javier
A1 Rodríguez-Bejarano, Eduardo
A1 Beuzón-López, Carmen del Rosario
K1 Receptores celulares
AB Plants encode numerous intracellular receptors known as nucleotide-binding leucine-rich repeat receptors (NLRs)that recognize pathogen-derived effectors or their activity to activate defenses. miRNAs regulate NLR genes in manyspecies, often triggering the production of phased siRNAs (phasiRNAs). Most such examples involve genes encodingNLRs carrying coiled-coil domains, although a few include genes encoding NLRs carrying a Toll/interleukin-1 domain(TNL). Here, we characterize the role of miR825-5p in Arabidopsis, using a combination of bioinformatics, transgenicplants with altered miRNA levels and/or reporters, small RNAs, and virulence assays. We demonstrate thatmiR825-5p down-regulates the TNL MIST1 by targeting for endonucleolytic cleavage the sequence coding for TIR2,a highly conserved amino acid motif, linked to a catalytic residue essential for immune function. miR825-5p acts asa negative regulator of basal resistance against Pseudomonas syringae. miR825-5p triggers the production fromMIST1 of a large number of phasiRNAs that can mediate cleavage of both MIST1 and additional TNL gene transcripts,potentially acting as a regulatory hub. miR825-5p is expressed in unchallenged leaves and transcriptionally downregulatedin response to pathogen-associated molecular patterns (PAMPs). Our results show that miR825-5p, whichis required for full expression of PAMP-triggered immunity, establishes a link between PAMP perception and expressionof uncharacterized TNL genes.
PB Oxford University Press
YR 2021
FD 2021-10-26
LK https://hdl.handle.net/10630/35705
UL https://hdl.handle.net/10630/35705
LA eng
NO López-Márquez D, Del-Espino Á, López-Pagán N, Rodríguez-Negrete EA, Rubio-Somoza I, Ruiz-Albert J, Bejarano ER, Beuzón CR. miR825-5p targets the TIR-NBS-LRR gene MIST1 and down-regulates basal immunity against Pseudomonas syringae in Arabidopsis. J Exp Bot. 2021 Oct 26;72(20):7316-7334
NO Ministerio de Economia y Competitividad (BIO2015-64391-R) (RYC-2015–19154) (BFU2014-58361-JIN)Ministerio de Ciencia, Innovación y Universidades (RTI2018-095069-B-100) (RTI2018-097262-B-I00)Junta de Andalucía Proyecto Operativo FEDER Andalucía (UMA18-FEDERJA-070)Formación de Profesorado Universitario (FPU14/04233) (FPU17/03520)
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 19 ene 2026