RT Book, Section
T1 Mapping Functional Connectivity in the Rodent Brain Using Electric-Stimulation fMRI.
A1 Pérez-Cervera, Laura
A1 Caramés-Tejedor, José María
A1 Fernández-Mollá, Luis Miguel
A1 Moreno, Andrea
A1 Fernández, Begoña
A1 Pérez-Montoyo, Elena
A1 Moratal, David
A1 Canals-Gamoneda, Santiago
A1 Pacheco-Torres, Jesús
A2 García-Martín, María Luisa
A2 López-Larrubia, Pilar
K1 Resonancia magnética nuclear (Medicina) - Modelos animales
AB Since its discovery in the early 90s, BOLD signal-based functional Magnetic Resonance Imaging (fMRI) has become a fundamental technique for the study of brain activity in basic and clinical research. Functional MRI signals provide an indirect but robust and quantitative readout of brain activity through the tight coupling between cerebral blood flow and neuronal activation, the so-called neurovascular coupling. Combined with experimental techniques only available in animal models, such as intracerebral micro- stimulation, optogenetics or pharmacogenetics, provides a powerful framework to investigate the impact of specific circuit manipulations on overall brain dynamics. The purpose of this chapter is to provide a comprehensive protocol to measure brain activity using fMRI with intracerebral electric micro-stimulation in murine models. Preclinical research (especially in rodents) opens the door to very sophisticated and informative experiments, but at the same time imposes important constrains (i.e., anesthetics, translatability), some of which will be addressed here.
PB Springer Nature
SN 1064-3745
YR 2018
FD 2018
LK https://hdl.handle.net/10630/34797
UL https://hdl.handle.net/10630/34797
LA eng
NO Pérez-Cervera, L. et al. (2018). Mapping Functional Connectivity in the Rodent Brain Using Electric-Stimulation fMRI. In: García Martín, M., López Larrubia, P. (eds) Preclinical MRI. Methods in Molecular Biology, vol 1718. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-7531-0_8
NO Política de acceso abierto tomada de: https://www.springernature.com/gp/open-science/policies/book-policies
NO This work was supported by the Spanish Ministerio de Economía y Competitividad (MINECO) and FEDER funds under grants BFU2015-64380-C2-1-R (S.C.) and BFU2015-64380-C2-2-R(D.M.) and EU Horizon 2020 Program 668863-SyBil-AA grant (S.C.). S.C. acknowledges financial support from the Spanish State Research Agency, through the “Severo Ochoa” Programme for Centres of Excellence in R&D (ref. SEV- 2013-0317).
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 20 ene 2026