RT Journal Article
T1 Localization of peroxisome proliferator-activated receptor alpha (PPARα) and N-acyl phosphatidylethanolamine phospholipase D (NAPE-PLD) in cells expressing the Ca(2+)-binding proteins calbindin, calretinin, and parvalbumin in the adult rat hippocampus.
A1 Rivera-González, Patricia
A1 Arrabal, Sergio
A1 Vargas, Antonio
A1 Blanco-Calvo, Eduardo
A1 Serrano, Antonia
A1 Pavón, Francisco Javier
A1 Rodriguez-de-Fonseca, Fernando
A1 Suárez-Pérez, Juan
K1 Inmunoquímica
K1 Hipocampo (Cerebro)
K1 Microscopía confocal
K1 Proteínas transportadoras del calcio
K1 Modelos animales en investigación
AB The N-acylethanolamines (NAEs), oleoylethanolamide (OEA) and palmithylethanolamide (PEA) are known to be endogenous ligands of PPARα receptors, and their presence requires the activation of a specific phospholipase D (NAPE-PLD) associated with intracellular Ca(2+) fluxes. Thus, the identification of a specific population of NAPE-PLD/PPARα-containing neurons that express selective Ca(2+)-binding proteins (CaBPs) may provide a neuroanatomical basis to better understand the PPARα system in the brain. For this purpose, we used double-label immunofluorescence and confocal laser scanning microscopy for the characterization of the co-existence of NAPE-PLD/PPARα and the CaBPs calbindin D28k, calretinin and parvalbumin in the rat hippocampus. PPARα expression was specifically localized in the cell nucleus and, occasionally, in the cytoplasm of the principal cells (dentate granular and CA pyramidal cells) and some non-principal cells of the hippocampus. PPARα was expressed in the calbindin-containing cells of the granular cell layer of the dentate gyrus (DG) and the SP of CA1. These principal PPARα(+)/calbindin(+) cells were closely surrounded by NAPE-PLD(+) fiber varicosities. No pyramidal PPARα(+)/calbindin(+) cells were detected in CA3. Most cells containing parvalbumin expressed both NAPE-PLD and PPARα in the principal layers of the DG and CA1/3. A small number of cells containing PPARα and calretinin was found along the hippocampus. Scattered NAPE-PLD(+)/calretinin(+) cells were specifically detected in CA3. NAPE-PLD(+) puncta surrounded the calretinin(+) cells localized in the principal cells of the DG and CA1. The identification of the hippocampal subpopulations of NAPE-PLD/PPARα-containing neurons that express selective CaBPs should be considered when analyzing the role of NAEs/PPARα-signaling system in the regulation of hippocampal functions.
PB Frontiers
YR 2014
FD 2014-03
LK https://hdl.handle.net/10630/40139
UL https://hdl.handle.net/10630/40139
LA eng
NO Patricia Rivera, Sergio Arrabal, Antonio Vargas, Eduardo Blanco, Antonia Serrano, Francisco J Pavón, Fernando Rodríguez de Fonseca, Juan Suárez. Localization of peroxisome proliferator-activated receptor alpha (PPARα) and N-acyl phosphatidylethanolamine phospholipase D (NAPE-PLD) in cells expressing the Ca(2+)-binding proteins calbindin, calretinin, and parvalbumin in the adult rat hippocampus. Front Neuroanat . 2014 Mar 17:8:12. doi: 10.3389/fnana.2014.00012.
DS RIUMA. Repositorio Institucional de la Universidad de Málaga
RD 20 ene 2026